Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Jul;51(7):2396-9.
doi: 10.1128/JCM.00151-13. Epub 2013 Apr 24.

Integrating the Xpert MTB/RIF assay into a diagnostic workflow for rapid detection of Mycobacterium tuberculosis in a low-prevalence area

Vanessa Deggim  1 , Akos Somoskovi, Antje Voit, Erik C Böttger, Guido V Bloemberg
Affiliations

Integrating the Xpert MTB/RIF assay into a diagnostic workflow for rapid detection of Mycobacterium tuberculosis in a low-prevalence area

Vanessa Deggim et al. J Clin Microbiol. 2013 Jul.

Abstract

The Xpert MTB/RIF assay is a rapid and fully automated real-time PCR assay. The performance of the Xpert MTB/RIF assay as a primary screening test for urgent clinical specimens was evaluated during a 2-year period. The results showed that replacing smear microscopy with the Xpert MTB/RIF assay facilitates laboratory handling and improves the sensitivity and specificity of Mycobacterium tuberculosis detection.

PubMed Disclaimer

Comment in

References

    1. Steingart KR, Ramsay A, Pai M. 2007. Optimizing sputum smear microscopy for the diagnosis of pulmonary tuberculosis. Expert Rev. Anti Infect. Ther. 5:327–331 - PubMed
    1. Böddinghaus B, Rogall T, Flohr T, Blöcker H, Böttger EC. 1990. Detection and identification of mycobacteria by amplification of rRNA. J. Clin. Microbiol. 28:1751–1759 - PMC - PubMed
    1. Kirschner P, Rosenau J, Springer B, Teschner K, Feldmann K, Böttger EC. 1996. Diagnosis of mycobacterial infections by nucleic acid amplification: 18-month prospective study. J. Clin. Microbiol. 34:304–312 - PMC - PubMed
    1. Reischl U, Lehn N, Wolf H, Naumann L. 1998. Clinical evaluation of the automated Cobas Amplicor MTB assay for testing respiratory and nonrespiratory specimens. J. Clin. Microbiol. 36:2853–2860 - PMC - PubMed
    1. Piersimoni C, Scarparo C. 2003. Relevance of commercial amplification methods for direct detection of Mycobacterium tuberculosis complex in clinical samples. J. Clin. Microbiol. 41:5355–5365 - PMC - PubMed

Publication types

MeSH terms

LinkOut - more resources