Bovine cumulus cells protect maturing oocytes from increased fatty acid levels by massive intracellular lipid storage
- PMID: 23616596
- DOI: 10.1095/biolreprod.112.106062
Bovine cumulus cells protect maturing oocytes from increased fatty acid levels by massive intracellular lipid storage
Abstract
Metabolic conditions characterized by elevated free fatty acid concentrations in blood and follicular fluid are often associated with impaired female fertility. Especially elevated saturated fatty acid levels can be lipotoxic for several somatic cell types. The aim of this study was to determine the impact of elevated free fatty acid concentrations in follicular fluid on neutral lipids (fatty acids stored in lipid droplets) inside cumulus cells and oocytes and their developmental competence. To this end, cows were exposed to a short-term fasting period during final oocyte maturation. This resulted in elevated, but distinct, free fatty acid concentrations in blood and follicular fluid and a rise in the concentrations of in particular fatty acids with a chain length of 14-18 carbon atoms. Interestingly, elevated free fatty acid concentrations in follicular fluid resulted in a massive increase in the level of neutral lipids in cumulus cells, whereas the level of neutral lipid in oocytes was hardly affected. Furthermore, competence of oocytes to develop to the blastocyst stage after fertilization and culture of cumulus-oocyte-complexes of the experimental and control group was not different. In conclusion these data suggest that short-term elevated free fatty acid concentrations in follicular fluid do not harm oocyte developmental competence. We propose that the involvement of high levels of mobilized oleic acid in follicular fluid in combination with the induced lipid storage in cumulus cells serves to prevent harmful saturated fatty acid exposure to the oocyte.
Keywords: IVF; IVM; cows; embryo culture; in vitro fertilization; in vitro maturation; oocyte-follicle interactions; ruminants.
Similar articles
-
Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro.Reproduction. 2005 Oct;130(4):485-95. doi: 10.1530/rep.1.00735. Reproduction. 2005. PMID: 16183866
-
The cumulus cell layer protects the bovine maturing oocyte against fatty acid-induced lipotoxicity.Biol Reprod. 2015 Jan;92(1):16. doi: 10.1095/biolreprod.114.120634. Epub 2014 Oct 8. Biol Reprod. 2015. PMID: 25297544
-
Comparative importance of fatty acid beta-oxidation to nuclear maturation, gene expression, and glucose metabolism in mouse, bovine, and porcine cumulus oocyte complexes.Biol Reprod. 2013 May 2;88(5):111. doi: 10.1095/biolreprod.113.108548. Print 2013 May. Biol Reprod. 2013. PMID: 23536372
-
Lipids and oocyte developmental competence: the role of fatty acids and β-oxidation.Reproduction. 2014 Jul;148(1):R15-27. doi: 10.1530/REP-13-0251. Epub 2014 Apr 23. Reproduction. 2014. PMID: 24760880 Review.
-
Lipid Metabolism in Bovine Oocytes and Early Embryos under In Vivo, In Vitro, and Stress Conditions.Int J Mol Sci. 2021 Mar 26;22(7):3421. doi: 10.3390/ijms22073421. Int J Mol Sci. 2021. PMID: 33810351 Free PMC article. Review.
Cited by
-
Melatonin Modulates Lipid Metabolism in Porcine Cumulus-Oocyte Complex via Its Receptors.Front Cell Dev Biol. 2021 Apr 1;9:648209. doi: 10.3389/fcell.2021.648209. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 33869202 Free PMC article.
-
Bovine In Vitro Oocyte Maturation and Embryo Production Used as a Model for Testing Endocrine Disrupting Chemicals Eliciting Female Reproductive Toxicity With Diethylstilbestrol as a Showcase Compound.Front Toxicol. 2022 May 24;4:811285. doi: 10.3389/ftox.2022.811285. eCollection 2022. Front Toxicol. 2022. PMID: 35686045 Free PMC article.
-
A primary effect of palmitic acid on mouse oocytes is the disruption of the structure of the endoplasmic reticulum.Reproduction. 2021 Dec 28;163(1):45-56. doi: 10.1530/REP-21-0332. Reproduction. 2021. PMID: 34866595 Free PMC article.
-
High-Fat Diet and Female Fertility across Lifespan: A Comparative Lesson from Mammal Models.Nutrients. 2022 Oct 17;14(20):4341. doi: 10.3390/nu14204341. Nutrients. 2022. PMID: 36297035 Free PMC article. Review.
-
Proteomic changes in oocytes after in vitro maturation in lipotoxic conditions are different from those in cumulus cells.Sci Rep. 2019 Mar 6;9(1):3673. doi: 10.1038/s41598-019-40122-7. Sci Rep. 2019. PMID: 30842615 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources