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. 2013 Jul;94(1):22-4.
doi: 10.1016/j.mimet.2013.04.005. Epub 2013 Apr 23.

Polymerase chain reaction with insertion sequence-specific and -unrelated primers: a new tool for the identification of IS1548 insertion targets in Streptococcus agalactiae

Maud Fléchard  1 , Agnès Rosenau, Laurent Mereghetti, Philippe Gilot
Affiliations

Polymerase chain reaction with insertion sequence-specific and -unrelated primers: a new tool for the identification of IS1548 insertion targets in Streptococcus agalactiae

Maud Fléchard et al. J Microbiol Methods. 2013 Jul.

Abstract

We developed a PCR method with outward insertion sequence-specific and -unrelated primers to identify IS1548 targets in the genome of unsequenced Streptococcus agalactiae strains. Our rapid and easy method allowed the identification of previously known but also of yet unnoticed integration sites in the three clinical isolates tested.

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