Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1975 May;122(2):385-92.
doi: 10.1128/jb.122.2.385-392.1975.

Autolysis of Neisseria gonorrhoeae

B H Hebeler, F E Young

Autolysis of Neisseria gonorrhoeae

B H Hebeler et al. J Bacteriol. 1975 May.

Abstract

Physiological conditions that would provide maximal rates of autolysis of Neisseria gonorrhoeae were examined. Autolysis was found to occur over a broad pH range with the optimum at pH 9.0 IN 0.05 M tris(hydroxymethyl)amino-methane-maleate buffer. The temperature optimum was found to be 40 C. Potassium ions greatly stimulated autolysis at a concentration of 0.01 M. Exposure of growing N. gonorrhoeae cells to penicillin, vancomycin, or D-cycloserine influenced the susceptibility to the autolysis, whereas chloramphenicol afforded some protection against autolysis. The primary structure of the peptidoglycan is composed of muramic acid/glutamic acid/alanine/diaminopimelic acid/glucosamine in approximate molar ratios of 1:1:2:1:1, respectively. Exogenous radioactive diaminopimelic acid, D-glucosamine, and D-alanine were incorporated into peptidoglycan. During autolysis these radioactive fragments were released from cells.

PubMed Disclaimer

References

    1. Adv Enzymol Relat Areas Mol Biol. 1964;26:193-232 - PubMed
    1. Z Naturforsch B. 1963 Nov;18:950-6 - PubMed
    1. Bacteriol Rev. 1968 Dec;32(4 Pt 2):425-64 - PubMed
    1. Nature. 1970 Jul 11;227(5254):138-40 - PubMed
    1. Eur J Biochem. 1973 Oct 5;38(2):301-6 - PubMed

Publication types

LinkOut - more resources