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. 2013 Oct;15(5):552-9.
doi: 10.1007/s11307-013-0639-6.

Synchrotron radiation X-ray phase micro-computed tomography as a new method to detect iron oxide nanoparticles in the brain

M Marinescu  1 , M Langer, A Durand, C Olivier, A Chabrol, H Rositi, F Chauveau, T H Cho, N Nighoghossian, Y Berthezène, F Peyrin, M Wiart
Affiliations

Synchrotron radiation X-ray phase micro-computed tomography as a new method to detect iron oxide nanoparticles in the brain

M Marinescu et al. Mol Imaging Biol. 2013 Oct.

Abstract

Purpose: The purpose of this study was to introduce synchrotron radiation X-ray phase computed tomography (SR-PCT) as a new method of visualizing ultrasmall superparamagnetic particles of iron oxide (USPIO) distribution into the brains of mice with neuroinflammation.

Procedures: The sensitivity of the technique was assessed by performing back-to-back SR-PCT and magnetic resonance imaging (MRI) in mice stereotaxically injected with a range of USPIO concentrations. Eight mice with cerebral ischemia were then intravenously injected with USPIOs and imaged back-to-back with MRI and SR-PCT.

Results: SR-PCT proved sensitive enough to detect iron in nanomolar quantities. In stroke-induced animals, SR-PCT showed hyperintense areas in the regions of MR signal loss and immunostaining for macrophages. SR-PCT, moreover, identified brain anatomy as clearly as histology, without the need for sectioning or staining, with an examination time of 44 min per brain at an isotropic spatial resolution of 8 μm.

Conclusion: SR-PCT has potential for cellular imaging in intact brain, with unequaled neuroanatomy.

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