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. 2013 Jan;4(1):181-6.
doi: 10.4103/0976-9668.107287.

Role of Imipenem-resistant metallo-beta-lactamase positive pseudomonas aeruginosa carriers in nosocomial infections

Babu K V Yogeesha  1 , Amruta Kumari, Arun Kumar, Kumar K G Raghu
Affiliations

Role of Imipenem-resistant metallo-beta-lactamase positive pseudomonas aeruginosa carriers in nosocomial infections

Babu K V Yogeesha et al. J Nat Sci Biol Med. 2013 Jan.

Abstract

Background: Imipenem-resistant metallo-beta-lactamase Positive Pseudomonas aeruginosa (IR-MBLP-PA) infections occur as outbreaks and epidemics with a potential to spread within and between hospitals and intercontinentally. Limited data is available on IR-MBLP-PA carriers and their role as source and/or reservoir of nosocomial infection.

Objectives: Detection and antibiogram typing of IR-MBLP-PA from healthy healthcare workers (HCW) from different areas of hospital and to assess role of carriers as source and/or reservoir of nosocomial infections.

Material and methods: Specimens from 200 HCWs [ICUs (120), General wards (40) and OPDs (40)] were collected from axilla, hands, stool and throat and processed by standard laboratory procedures. IR-MBLP-PA detection is done by IMIPENEM+EDTA combined disc test. Antibiogram typing is done. Association of carriers with clinical cases is done by IR-MBLP-PA with identical antibiogram type from carriers and cases. Distribution of carriers was assessed by Chi-square test.

Results: Incidence of P. aeruginosa and IR-MBLP-PA carriers among HCWs was 25%, 3.21% in ICUs, 10% from general wards and 0% from OPDs. A total of five IR-MBLP-PA antibiogram types were observed from four carriers and none from general wards and OPDs. Distribution of P. aeruginosa and IR-MBLP-PA carriers in different areas of hospital was not statistically significant with P values of 0.058 and 0.76, respectively.

Conclusions: Role of IR-MBLP-PA carriers as source and/or reservoirs of infections could not be assessed with certainty; however, the possibility cannot be ruled out. Periodic carrier studies in targeted high risk areas of hospital should be undertaken.

Keywords: Carriers; Healthcare workers; Imipenem-resistant metallo-beta-lactamase positive P. aeruginosa (IR-MBLP-PA).

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Conflict of interest statement

Conflict of Interest: None declared

References

    1. Kurokawa H, Yagi T, Shibata N, Arakawa Y. Worldwide proliferation of Carbapenem resistant Gram negative bacteria. Lancet. 1999;354:955. - PubMed
    1. Walsh TR, Toleman MA, Poirel L, Nordmann P. Metallo-beta lacrtamases. The quiet before the storm? Clin Microbiol Rev. 2005;18:306–25. - PMC - PubMed
    1. Laupland KB, Parkins MD, Church DL. Population-based epidemiological study of infections caused by carbapenem-resistant Pseudomonas aeruginosa in the Calgary health region: Importance of metallo-beta-lactamase (MBL) producing strains. J Infect Dis. 2005;192:1606–12. - PubMed
    1. Wright GD, Sutherland AD. New strategies for combating multidrug-resistant bacteria. Trends Mol Med. 2007;13:260–7. - PubMed
    1. Performance standards for antimicrobial susceptibility testing. 17 Suppl. Vol. 27. Wayne, PA: Clinical Laboratory Standards; 2007. Clinical and Laboratory Standards Institute.

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