Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Apr 18;3(2):122-4.
Print 2013.

Detection of bla(IMP) and bla(VIM) metallo-β-lactamases genes among Pseudomonas aeruginosa strains

Fatemeh Fallah  1 , Rebwar Shams Borhan, Ali Hashemi
Affiliations

Detection of bla(IMP) and bla(VIM) metallo-β-lactamases genes among Pseudomonas aeruginosa strains

Fatemeh Fallah et al. Int J Burns Trauma. .

Abstract

Acquired Metallo-β-Lactamases (MBLs) are emerging resistance determinants in Pseudomonas aeruginosa and other gram-negative bacteria.Using Combination Disk Diffusion test, it was found that among 83 imipenem non-susceptible P. aeruginosa strains, 48 (57.9%) were MBL producers. PCR and Sequencing methods proved that these isolates were positive for blaIMP-1 genes, whereas none were positive for bla(VIM) genes. The mortality rate due to MBL-producing Pseudomonas infection was 4 (8.3%) among the hospitalized patients. Therefore, identification of drug resistance patterns in P. aeruginosa and detection of MBLs producing isolates are of great importance in the prevention and control of infections.

Keywords: P. aeruginosa; antibiotics; metallo-β-lactamases; mortality.

PubMed Disclaimer

Figures

Figure 1
Figure 1
MBL screening by CDDT test showed that 48 (57.9%) of imipenem-nonsusceptible isolates were MBL positive. A MEM disc, B MEM 10μg+EDTA 10 μg disc, C IMI 10 μg disc and D IMI10μg+EDTA 10 μg disc.
See this image and copyright information in PMC

References

    1. Tawfik AF, Shibl AM, Aljohi MA, Altammami MA, Al-Agamy MH. Distribution of Ambler class A, B and D β-lactamases among Pseudomonas aeruginosa isolates. Burns. 2012 Sep;38:855–60. - PubMed
    1. Lee NY, Yan JJ, Lee HC, Liu KH, Huang ST, Ko WC. Clinical experiences of bacteremia caused by metallo-beta-lactamase-producing gram-negative organisms. J Microbiol Immunol Infect. 2004 Dec;37:343–9. - PubMed
    1. Bahar MA, Jamali S, Samadikuchaksaraei A. Imipenem-resistant Pseudomonas aeruginosa strains carry metallo-beta-lactamase gene bla(VIM) in a level I Iranian burn hospital. Burns. 2010 Sep;36:826–30. - PubMed
    1. Galani I, Rekatsina PD, Hatzaki D, Plachouras D, Souli M, Giamarellou H. Evaluation of different laboratory tests for the detection of metallo-beta-lactamase production in Enterobacteriaceae. J Antimicrob Chemother. 2008 Mar;61:548–53. - PubMed
    1. Khosravi AD, Mihani F. Detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients in Ahwaz, Iran. Diagn Microbiol Infect Dis. 2008 Jan;60:125–8. - PubMed

LinkOut - more resources