Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jun;10(2):227-35.
doi: 10.1007/BF00734576.

Differential regulation by dexamethasone of glucocorticoid receptor messenger RNA concentrations in neuronal cultures derived from fetal rat hypothalamus and cerebral cortex

M C Pepin  1 , S Beaulieu, N Barden
Affiliations

Differential regulation by dexamethasone of glucocorticoid receptor messenger RNA concentrations in neuronal cultures derived from fetal rat hypothalamus and cerebral cortex

M C Pepin et al. Cell Mol Neurobiol. 1990 Jun.

Abstract

1. Differential regulation, by dexamethasone, of glucocorticoid receptor gene expression was studied in three different neuronal cultures derived from hypothalamus amygdala, and cerebral cortex. 2. Cellular glucocorticoid receptor (GR) mRNA concentration was measured by hybridization using a 32P-labeled RNA probe complementary to a 2.2-kb fragment of the glucocorticoid receptor mRNA. Changes in the amount of GR mRNA were evaluated in relation to the content of beta-actin mRNA. 3. In cells derived from either hypothalamus or cerebral cortex, we observed a complex pattern of GR mRNA concentrations which were characterized by cyclic variations of GR mRNA content during continuous treatment with dexamethasone for up to 72 hr. 4. In contrast to cells derived from the hypothalamus where a persistent 30-40% reduction in GR mRNA levels was seen for up to a least 72 hr, we observed, in cells derived from the cerebral cortex, a sustained increased (1.4-fold) of the GR mRNA at this same time interval.

PubMed Disclaimer

References

    1. Angelucci, L., and Patacchioli, F. R. (1985). InStress: The Role of Catecholamines and Other Neurotransmitters (E. Usdin, R. Kvetnansky, and J. Axelrod, Eds.) Gordon & Breach Science, New York; pp. 731–740.
    1. Dianielse, M., and Stallcup, M. R. (1984). Down-regulation of glucocorticoid receptors in mouse lymphoma cell variants.Moll. Cell. Biol.4449–453. - PMC - PubMed
    1. Faivre-Bauman, A., Puymirat, J., Loudes, C., and Tixier-Vidal, A. (1984). Differentiated mouse fetal hypothalamic cells in serum-free medium. InMethods for Serum-Free Culture of Neuronal and Lymphoid Cells, Alan R. Liss, New York, pp. 37–56.
    1. Feldman, S. (1985). Neural pathways mediating adrenocortical responses.Fed. Proc.44169–175. - PubMed
    1. Feldman, S., and Conforti, N. (1985). Modifications of adrenocortical responses following frontal cortex stimulation in rats with hypothalamic deafferentations and medial forebrain bundle lesions.Neuroscience151045–1047. - PubMed

MeSH terms

LinkOut - more resources