Macro-invertebrate decline in surface water polluted with imidacloprid

Abstract

Imidacloprid is one of the most widely used insecticides in the world. Its concentration in surface water exceeds the water quality norms in many parts of the Netherlands. Several studies have demonstrated harmful effects of this neonicotinoid to a wide range of non-target species. Therefore we expected that surface water pollution with imidacloprid would negatively impact aquatic ecosystems. Availability of extensive monitoring data on the abundance of aquatic macro-invertebrate species, and on imidacloprid concentrations in surface water in the Netherlands enabled us to test this hypothesis. Our regression analysis showed a significant negative relationship (P<0.001) between macro-invertebrate abundance and imidacloprid concentration for all species pooled. A significant negative relationship was also found for the orders Amphipoda, Basommatophora, Diptera, Ephemeroptera and Isopoda, and for several species separately. The order Odonata had a negative relationship very close to the significance threshold of 0.05 (P = 0.051). However, in accordance with previous research, a positive relationship was found for the order Actinedida. We used the monitoring field data to test whether the existing three water quality norms for imidacloprid in the Netherlands are protective in real conditions. Our data show that macrofauna abundance drops sharply between 13 and 67 ng l(-1). For aquatic ecosystem protection, two of the norms are not protective at all while the strictest norm of 13 ng l(-1) (MTR) seems somewhat protective. In addition to the existing experimental evidence on the negative effects of imidacloprid on invertebrate life, our study, based on data from large-scale field monitoring during multiple years, shows that serious concern about the far-reaching consequences of the abundant use of imidacloprid for aquatic ecosystems is justified.

Figure 1. Relationship between log₁₀ imidacloprid concentration and log₁₀ Amphipoda and Actinedida abundance in surface water.

a) Amphipoda (P = <0.001), b) its most abundant species Gammarus tigrinus (P = 0.001), c) Actinedida (P = <0.001), d) its most abundant species Limnesia undulata (P = 0.022).

Figure 2. Macro-invertebrate abundance in surface water samples below and above Dutch imidacloprid norms for surface water.

Mean and standard error of abundance is shown. We used median imidacloprid concentrations. Dependent variables were tested separately using the Mann-Whitney test. *Indicates significant differences at P<0.05. MTR = Maximum Permissible Risk imidacloprid, MPC = Maximum Permissible Concentration imidacloprid, MAC = Maximum Acceptable Concentration imidacloprid (see text).