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. 1990 Jun 18;266(1-2):59-62.
doi: 10.1016/0014-5793(90)81506-j.

A protein conformational change associated with the photoreduction of the primary and secondary quinones in the bacterial reaction center

E Nabedryk  1 , K A Bagley, D L Thibodeau, M Bauscher, W Mäntele, J Breton
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Free article

A protein conformational change associated with the photoreduction of the primary and secondary quinones in the bacterial reaction center

E Nabedryk et al. FEBS Lett. .
Free article

Abstract

A comparison is made between the PQA----P+QA- and PQAQB----P+QAQB-transitions in Rps. viridis and Rb. sphaeroides reaction centers (RCs) by the use of light-induced Fourier transform infrared (FTIR) difference spectroscopy. In Rb. sphaeroides RCs, we identify a signal at 1650 cm-1 which is present in the P+QA-minus-PQA spectrum and not in the P+QAQB(-)-minus-PQAQB spectrum. In contrast, this signal is present in both P+QA(-)-minus-PQA- and P+QAQB(-)-minus-PQAQB spectra of Rps. viridis RCs. These data are interpreted in terms of a conformational change of the protein backbone near QA (possible at the peptide C = O of a conserved alanine residue in the QA pocket) and of the different bonding interactions of QB with the protein in the RC of the two species.

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