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. 2013 Jun;30(6):835-41.
doi: 10.1007/s10815-013-0007-0. Epub 2013 May 9.

Effect of micro-vibration culture system on embryo development

Yong Soo Hur  1 , Jeong Hyun Park, Eun Kyung Ryu, Sung Jin Park, Jun Ho Lee, Soo Hee Lee, Jung Yoon, San Hyun Yoon, Chang Young Hur, Won Don Lee, Jin Ho Lim
Affiliations

Effect of micro-vibration culture system on embryo development

Yong Soo Hur et al. J Assist Reprod Genet. 2013 Jun.

Abstract

Purpose: Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders.

Materials and methods: The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (n = 178) and a micro-vibration culture group (n = 181) in mice. The embryonic development rates and clinical results were compared between a static culture group (n = 159 cycles) and a micro-vibration culture group (n = 166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.

Results: The embryonic development rate was significantly improved in the micro-vibration culture group in mice (p < 0.05). The cell numbers of mouse blastocysts were significantly higher in the micro-vibration group than in the static culture group (p < 0.05). In the poor responders, the rate of high grade embryos was not significantly improved in the micro-vibration culture group on day 3. However, the optimal embryonic development rate on day 5 was improved in the micro-vibration group, and the total pregnancy rate and implantation rate were significantly higher in the micro-vibration group than in the static culture group (p < 0.05).

Conclusions: Micro-vibration culture methods have a beneficial effect on embryonic development in mouse embryos. In poor responders, the embryo development rate was improved to a limited extent under the micro-vibration culture conditions, but the clinical results were significantly improved.

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Figures

Fig. 1
Fig. 1
a Appearance of the micro-vibrator installed in the incubator. b 3-dimensional movement of the micro-vibrator (according to the product specifications provided by Nepa Gene)
Fig. 2
Fig. 2
Comparison of mouse embryo quality between the static culture group and the micro-vibration culture group on day 3 (a, b) and day 5 (c, d) and a comparison of the cell numbers between the static culture group and the micro-vibration culture group (e, f) a Static cultured embryos on day 3, magnification ×200 b Micro-vibration cultured embryos on day 3, magnification ×200 c Static cultured embryos on day 5, magnification ×200 d Micro-vibration cultured embryos on day 5, magnification ×200 e Stained blastocyst from the static culture, magnification ×400 f Stained blastocyst from the micro-vibration culture, magnification ×400
Fig. 3
Fig. 3
Comparison of the average cell numbers of mouse blastocysts between the static culture group and the micro-vibration culture group. Error bars represent the standard error of the mean
See this image and copyright information in PMC

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