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. 1990 Jul;86(1):356-62.
doi: 10.1172/JCI114709.

Growth stimulation by coexpression of transforming growth factor-alpha and epidermal growth factor-receptor in normal and adenomatous human colon epithelium

S D Markowitz  1 , K Molkentin, C Gerbic, J Jackson, T Stellato, J K Willson
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Growth stimulation by coexpression of transforming growth factor-alpha and epidermal growth factor-receptor in normal and adenomatous human colon epithelium

S D Markowitz et al. J Clin Invest. 1990 Jul.

Abstract

Autocrine stimulation of the epidermal growth factor receptor (EGF-R), by coexpression of transforming growth factor-alpha (TGF-alpha), causes malignant transformation of some fibroblast cell lines. TGF-alpha and EGF-R are both known to be expressed in colon carcinoma tissue and have been shown coexpressed in colon carcinoma cell lines. TGF-alpha autocrine activation of EGF-R has been suggested as a potential mechanism contributing to abnormal growth control in colon cancer. We now report coexpression of TGF-alpha and EGF-R transcripts in morphologically normal colonic epithelium from five individuals, in colonic adenomas from three individuals, and in a nontumorigenic colon adenoma cell line, VACO-330. Functional studies demonstrate VACO-330 growth is stimulated by exogenous TGF-alpha and is completely abolished by a blocking anti-EGF-R antibody. Autocrine stimulation of EGF-R by TGF-alpha is therefore required for growth of the adenoma cell line. Autocrine stimulation of EGF-R by TGF-alpha does not cause malignant transformation of the colonic epithelial cell. In normal and adenomatous human colon TGF-alpha, via either an autocrine or paracrine mechanism, is likely an important physiologic stimulant of epithelial proliferation.

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References

    1. Cancer Lett. 1988 May;40(1):59-70 - PubMed
    1. J Clin Invest. 1989 Nov;84(5):1683-7 - PubMed
    1. Nature. 1990 Jan 4;343(6253):82-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1978 Aug;75(8):4001-5 - PubMed
    1. N Engl J Med. 1980 Oct 9;303(15):878-80 - PubMed

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