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Review

General Spinfection Protocol

W Travis Berggren  1 , Margaret Lutz  1 , Veronica Modesto  1
In: StemBook [Internet]. Cambridge (MA): Harvard Stem Cell Institute; 2008.
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Review

General Spinfection Protocol

W Travis Berggren et al.
Free Books & Documents

Excerpt

This protocol was developed in the Salk STEM Cell Core to enable researchers to consistently and reproducibly produce reprogrammed iPS cells, the initial idea came via word of mouth reports of its effectiveness to increase the efficiency of viral transduction. It has most commonly been used on retrovirus and lentivirus factors. Initial evaluation of this method showed 5–10× increase in transduction efficiency.

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