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Review

Neural induction – Dual SMAD inhibition

Mark Tomishima  1
In: StemBook [Internet]. Cambridge (MA): Harvard Stem Cell Institute; 2008.
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Review

Neural induction – Dual SMAD inhibition

Mark Tomishima.
Free Books & Documents

Excerpt

Dual SMAD inhibition takes a confluent, feeder free culture of hPSCs and rapidly differentiates them into early neurectoderm (Chambers et al., 2009). This rapid differentiation is caused by blocking the two signaling pathways that utilize SMADs for transduction: BMP and TGFB. Oct4 is extinguished and Pax6 expression has begun by around day 7–8, depending on the line used. This neurectoderm can be passaged to become rosettes or can be patterning to become many other types of neural cells (for example, see Fasano et al., 2010).

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References

    1. Chambers S.M, Craft C.A, Papapetrou E.P, Tomishima M, Sadelain M, Studer L. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 2009;27(3):275–280. Epub 2009 Mar 1. Erratum in: Nat Biotechnol. 2009 May;27(5):485. doi: 10.1038/nbt.1529. - DOI - PMC - PubMed
    1. Fasano C.A, Chambers S.M, Lee G, Tomishima M.J, Studer L. Efficient derivation of functional floor plate tissue from human embryonic stem cells. Cell Stem Cell. 2010;6(4):336–347. doi: 10.1016/j.stem.2010.03.001. - DOI - PMC - PubMed

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