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. 1977 Nov;21(6):463-8.

The effect of supraoptimal temperature on the formation of pseudorabies virus particles

F Golais, F Ciampor, A Sabó
  • PMID: 23664

The effect of supraoptimal temperature on the formation of pseudorabies virus particles

F Golais et al. Acta Virol. 1977 Nov.

Abstract

Replication of virulent and attenuated strains of pseudorabies virus (PRV) at supraoptimal temperature was inhibited in L cells non-adapted to 40 degrees C. Electron microscopy revealed the prevalence of hollow core particles without nucleoid; both the nuclei and cytoplasm of L cells were markedly altered at supraoptimal temperature. In BHK-21 cells adapted to 40 degrees C only replication of attenuated strains was inhibited whereas the virulent ones reached high titres at this temperature. The particles produced at 40 degrees C by attenuated strains were non-infectious but morphologically normal. The ability of attenuated PRV strains to reproduce in adapted BHK-21 cells at 40 degrees C was in correlation with the degree of their attenuation. Changes in the nuclei and cytoplasm of adapted BHK-21 cells at supraoptimal temperature were not as marked as in L cells.

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