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. 2013 May 13;11(5):1534-52.
doi: 10.3390/md11051534.

Synthesis, characterization, and antibacterial activity of cross-linked chitosan-glutaraldehyde

Bin Li  1 , Chang-Lin Shan, Qing Zhou, Yuan Fang, Yang-Li Wang, Fei Xu, Li-Rong Han, Muhammad Ibrahim, Long-Biao Guo, Guan-Lin Xie, Guo-Chang Sun
Affiliations

Synthesis, characterization, and antibacterial activity of cross-linked chitosan-glutaraldehyde

Bin Li et al. Mar Drugs. .

Abstract

This present study deals with synthesis, characterization and antibacterial activity of cross-linked chitosan-glutaraldehyde. Results from this study indicated that cross-linked chitosan-glutaraldehyde markedly inhibited the growth of antibiotic-resistant Burkholderia cepacia complex regardless of bacterial species and incubation time while bacterial growth was unaffected by solid chitosan. Furthermore, high temperature treated cross-linked chitosan-glutaraldehyde showed strong antibacterial activity against the selected strain 0901 although the inhibitory effects varied with different temperatures. In addition, physical-chemical and structural characterization revealed that the cross-linking of chitosan with glutaraldehyde resulted in a rougher surface morphology, a characteristic Fourier transform infrared (FTIR) band at 1559 cm⁻¹, a specific X-ray diffraction peak centered at 2θ = 15°, a lower contents of carbon, hydrogen and nitrogen, and a higher stability of glucose units compared to chitosan based on scanning electron microscopic observation, FTIR spectra, X-ray diffraction pattern, as well as elemental and thermo gravimetric analysis. Overall, this study indicated that cross-linked chitosan-glutaraldehyde is promising to be developed as a new antibacterial drug.

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Figures

Figure 1
Figure 1
Effect of temperature on antibacterial activity of chitosan and cross-linked chitosan-glutaraldehyde (CLCG) against Burkholderia seminalis strain 0901. Data from the repeated experiment were pooled and subjected to analysis of variance. Columns with the same letters are not significantly different (p < 0.05). Error bars represent the standard error of the mean.
Figure 2
Figure 2
SEM images of cross-linked chitosan-glutaraldehyde (CLCG) at four different magnifications (a) 1500×; (b) 500×; (c) 250×; and (d) 200×.
Figure 3
Figure 3
FTIR spectra of (a) chitosan and (b) cross-linked chitosan-glutaraldehyde (CLCG).
Figure 4
Figure 4
X-ray diffractograms of (a) chitosan and (b) cross-linked chitosan-glutaraldehyde (CLCG).
Figure 5
Figure 5
Thermo gravimetric analysis of (a) chitosan and (b) cross-linked chitosan-glutaraldehyde (CLCG).
See this image and copyright information in PMC

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