Spectrophotometric determination of labetalol and lercanidipine in pure form and in pharmaceutical preparations using ferric-1,10-phenanthroline

Abstract

A simple and sensitive spectrophotometric method was developed for the determination of labetalol HCl (LBT) and lercanidipine HCl (LER) in pure form and in dosage forms. The method was based upon oxidation of the LBT and LER with Fe(+3) and the estimation of the produced Fe(+2) with 1,10-phenanthroline. The absorbance of the tris(1,10-phenanthroline) Fe(+2) complex was measured at 510 nm. Reaction conditions were optimized to obtain colored complex of higher sensitivity and longer stability. The absorbance concentration plots were rectilinear over the concentration rang of 5-90 and 1-20 μg/mL with lower detection limits of 0.74 and 0.01 μg/mL and quantification limits of 2.26 and 0.02 μg/mL for LBT and LER, respectively. The developed method was successfully applied for the determination of LBT and LER in bulk drugs and dosage forms. The common excipients and additives did not interfere in their determinations. There was no significant difference between the results obtained by the proposed and the reference methods regarding Student t-test and the variance ratio F-test.

Keywords: Ferric-1,10-Phenanthroline; labetalol; lercanidipine; spectrophotometry.

Figure 1

Proposal of the mechanism of reaction between labetalol and lercanidipine with ferric-1,10-phenanthroline.

Figure 2

Effect of different volumes of 0.01 M ferric 1,10-phenanthroline reagent on the development of the reaction products.

Figure 3

Effect of the heating temperature on the formation of the colored product.

Figure 4

Effect of boiling time on the development of the reaction products.