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. 2012 Dec;8(4):258-63.

(31)P and (1)h nuclear magnetic resonance spectroscopy of blood plasma in female patients with preeclampsia

Susanne Schott  1 , Josef Hahn, Christian Kurbacher, Detlef Moka
Affiliations

(31)P and (1)h nuclear magnetic resonance spectroscopy of blood plasma in female patients with preeclampsia

Susanne Schott et al. Int J Biomed Sci. 2012 Dec.

Abstract

Objective: Using (31)P and (1)H magnetic resonance spectroscopy to measure phosphorus- and hydrogen-containing metabolites, this study aimes to investigate whether or not women with preeclampsia have detectable systemic abnormalities concerning certain components of the blood plasma.

Methods: PLASMA WAS OBTAINED FROM TWO GROUPS OF WOMEN: Group 1 with preeclampsia (n=10) and Group 2, as a control group with no complications during pregnancy (n=10). Plasma analysis were performed using in-vitro (31)P and (1)H nuclear magnetic resonance spectroscopy.

Results: (31)P nuclear magnetic resonance (NMR) spectra showed significantly higher levels of lysophosphatidylcholine 1 in the plasma of the patients in Group 1, along with significantly decreased levels of lysophosphatidylcholine 2 and phosphatidylinositol. However, the total amount of phospholipids did not differ significantly between the groups. In addition, the (1)H NMR spectra showed a significantly lower level of HDL in samples from Group 1, and a trend towards higher plasma levels of VLDL 2 and LDL 2 in the same group.

Conclusion: This study supports the theory that preeclampsia is a disorder in phospholipid metabolism in which malfunctioning of cellular membranes seems to play a major pathogenic role.

Keywords: 31P and 1H-magnetic resonance spectroscopy; gestosis; lipid metabolism; preeclampsia.

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Figures

Figure 1
Figure 1
One-dimensional 31P NMR blood spectra in pregnant women. A, 31P NMR spectrum in a patient suffering from preeclampsia. B, 31P NMR spectrum in a healthy, pregnant woman (*shows significant differences in concentrations of substances). Chemical shift assignments were referenced relative to 85% orthophosphoric acid at 0 ppm.
Figure 2
Figure 2
Partial 1H NMR spectra in a pregnant women. A, 1H NMR spectrum in a patient suffering from preeclampsia. B, 1H NMR spectrum in a healthy, pregnant woman. The assignments of the major NMR resonance are given in Table 2. (Standard solution (Std.) tetradeuterotrimethylsilylpropionate).
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