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. 2013 Nov;133(11):2640-2642.
doi: 10.1038/jid.2013.190. Epub 2013 Apr 18.

Creation of differentiation-specific genomic maps of human epidermis through laser capture microdissection

Nicholas Gulati  1 , James G Krueger  2 , Mayte Suárez-Fariñas  3 , Hiroshi Mitsui  1
Affiliations

Creation of differentiation-specific genomic maps of human epidermis through laser capture microdissection

Nicholas Gulati et al. J Invest Dermatol. 2013 Nov.
No abstract available

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Conflict of interest statement

CONFLICT OF INTEREST: The authors state no conflict of interest.

Figures

Figure 1
Figure 1
Principal components analysis and immunohistochemistry confirm effective separation of different epidermal regions. (a) Principal components analysis of microarray data. The “Whole Epidermis” data points represent full epidermis LCM performed as part of a separate experiment (Kennedy-Crispin et al., 2012). (b–h) Immunohistochemical confirmation of transcription factor localization (see Table 1). Immunohistochemistry staining of normal skin samples for: (b) CD11c as a negative control for epidermal staining, (c) pituitary tumortr-ansforming gene 1 (PTTG1), (d) zinc finger protein 281 (ZNF281), (e) regulatory factor X, 5 (RFX5), (f) Sp4 transcription factor (SP4), (g) aryl hydrocarbon receptor nuclear translocator-like 2 (ARNTL2), and (h) sterol regulatory element-binding transcription factor 2 (SREBF2). Images c–f confirm a basal layer localization while images g–h confirm a suprabasal localization. Scale bar = 100 µm.
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