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. 2013 May 16:10:150.
doi: 10.1186/1743-422X-10-150.

Genetic diversity of HA1 domain of heammaglutinin gene of influenza A(H1N1)pdm09 in Tunisia

Awatef El Moussi  1 , Mohamed Ali Ben Hadj Kacem, Francisco Pozo, Juan Ledesma, Maria Teresa Cuevas, Inmaculada Casas, Amine Slim
Affiliations

Genetic diversity of HA1 domain of heammaglutinin gene of influenza A(H1N1)pdm09 in Tunisia

Awatef El Moussi et al. Virol J. .

Abstract

We present major results concerning isolation and determination of the nucleotide sequence of hemagglutinin (HA1) of the pandemic (H1N1)pdm09 influenza viruses found in Tunisia. Amino acid analysis revealed minor amino acid changes in the antigenic or receptor-binding domains. We found mutations that were also present in 1918 pandemic virus, which includes S183P in 4 and S185T mutation in 19 of 27 viruses analyzed from 2011, while none of the 2009 viruses carried these mutations. Also two specific amino acid differences into N-glycosylation sites (N288T and N276H) were detected. The phylogenetic analysis revealed that the majority of the Tunisian isolates clustered with clade A/St. Petersburg/27/2011 viruses characterized by D97N and S185T mutations. However it also reveals a trend of 2010 strains to accumulate amino acid variation and form new phylogenetic clade with three specific amino acid substitutions: V47I, E172K and K308E.

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Figures

Figure 1
Figure 1
Phylogenetic relationship of partial length HA sequences of influenza A(H1N1)pdm09 viruses in Tunisia. The tree was rooted with the vaccine strain A/California/07/2009 (boxed) as outgroup. Branch lengths are drawn to scale. Signature amino acid changes (H1 numbering) are annotated at the nodes of each cluster.
See this image and copyright information in PMC

References

    1. Skehel JJ, Wiley DC. Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin. Annu Rev Biochem. 2000;39:531–569. - PubMed
    1. Knossow M, Skehel JJ. Variation and infectivity neutralization in influenza. Immunology. 2006;119:1–7. doi: 10.1111/j.1365-2567.2006.02421.x. - DOI - PMC - PubMed
    1. Breschkin AM, Ahern J, White DO. Antigenic determinants of influenza virus hemagglutinin. VIII. Topography of the antigenic regions of influenza virus hemagglutinin determined by competitive radioimmunoassay with monoclonal antibodies. Virology. 1981;113:130–40. doi: 10.1016/0042-6822(81)90142-2. - DOI - PubMed
    1. Caton AJ, Raymond FL, Brownlee GG, Yewdell JW, Gerhard W. Antigenic variation in influenza virus. Biochem Soc Trans. 1983;11:435–41. - PubMed
    1. Caton AJ, Brownlee GG, Yewdell JW, Gerhard W. The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype) Cell. 1982;31:417–27. doi: 10.1016/0092-8674(82)90135-0. - DOI - PubMed

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