Double-Stranded RNA Viral Infection in Tehran Trichomonas vaginalis Isolates

Abstract

Background: Trichomonas vaginalis is a pathogenic protozoon and may be contaminated with dsRNA virus called Trichomonas vaginalis virus (TVV). The viral infection is an important factor for its pathogenesis and sensitivity to metronidazole. The presence of TVV is associated with qualitative and quantitative expression of cysteine proteinases and surface immunogenic; P270. The purpose of this study was to determine TVV frequency in T. vaginalis clinical isolates in Tehran, Iran.

Methods: The 46 T. vaginalis isolates were collected from Tehran Province and cultured in TYI-S-33 culture medium. Viral RNA was extracted and RT-PCR was done.

Results: Of 46 T. vaginalis isolates, 8 isolates (17.39%) were infected with TVV-1. There was not any association between patient age and TVV- infected T. vaginalis. There were 17.39% viral infection in T. vaginalis isolates which was lower than that reported by other researchers.

Conclusion: This is the first report on T. vaginalis isolates infection by TVV-1 in Iran.

Keywords: Trichomonas vaginalis; Viral infection; dsRNA virus.

Fig. 1

2% agarose gel electrophoresis of PCR products. Lane 1: 100 bp DNA ladder marker. Lanes 2, 3, 4 and 6: virus positive samples, lane 5: virus negative sample