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. 2013 Apr;107(3):136-40.
doi: 10.1179/2047773213Y.0000000085.

Diagnosis and molecular characterization of Trichomonas vaginalis in sex workers in the Philippines

Macario Ireneo P Queza  1 , Windell L Rivera
Affiliations

Diagnosis and molecular characterization of Trichomonas vaginalis in sex workers in the Philippines

Macario Ireneo P Queza et al. Pathog Glob Health. 2013 Apr.

Abstract

Trichomonas vaginalis is a pathogenic protozoon which causes the sexually transmitted infection, trichomoniasis. The absence or non-specificity of symptoms often leads to misdiagnosis of the infection. In this study, 969 samples consisting of vaginal swabs and urine were collected and screened from social hygiene clinics across the Philippines. Of the 969 samples, 216 were used for the comparative analysis of diagnostic tools such as wet mount microscopy, culture, and PCR utilizing universal trichomonad primers, TFR1/2 and species-specific primers, TVK3/7 and TV1/2. PCR demonstrated higher sensitivity of 100% compared to 77% of the wet mount. PCR primer set TVK3/7 and culture had the same and the best expected average performance [receiver-operating characteristic (ROC): 0·98]. Prevalence of infection in the sample population was 6·8%.

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Figure 1
Figure 1
Location of study sites across the Philippines.
See this image and copyright information in PMC

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