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. 2013 Mar;34(3):185-9.
doi: 10.3760/cma.j.issn.0253-2727.2013.03.001.

[Biocompatibility of polyethylene imine (PEI)-coated magnetic Fe₃O₄ nanoparticles in SHI-1 cells]

[Article in Chinese]
Xiao-li Chao  1 , Zi-xing Chen, Su-ning Chen, Jian-nong Cen, Xiao-fei Qi, Hong-jie Shen, Li Yao, Yuan-yuan Wang
Affiliations

[Biocompatibility of polyethylene imine (PEI)-coated magnetic Fe₃O₄ nanoparticles in SHI-1 cells]

[Article in Chinese]
Xiao-li Chao et al. Zhonghua Xue Ye Xue Za Zhi. 2013 Mar.

Abstract
in English, Chinese

Objective: To explore the feasibility of magnetic resonance cell imaging technology by using polyethylene imine (PEI)-coated magnetic nanoparticles of Fe₄O₄ (PEI-Fe₄O₄-MNPs) to track cell biology behavior.

Methods: Endocytic PEI-Fe₄O₄-MNPs in SHI-1 cells were observed by transmission electron microscopy (TEM) . Iron contents of nano-labeled cells were analyzed by inductively coupled plasma-atomic emission spectroscopy (ICP-AES) and Prussian blue staining. The proliferation ability of labeled cells was detected by cell counting kit-8 (CCK-8) assay; the differentiation and colony-forming abilities were also observed. SHI-1 cells without endocytosing PEI-Fe₄O₄-MNPs were used as control.

Results: Our data showed that PEI-Fe₄O₄-MNPs could label SHI-1 cells. The labeling efficiency depended on the nanoparticles' concentration and the duration of cells treating. Inhibition rates of SHI-1cells labeled by 60-100 μg Fe/ml PEI-Fe₄O₄-MNPs were much higher than of 5-50 μg Fe/ml ones following treating by 5-100 μg Fe/ml PEI-Fe₄O₄-MNPs for 48 hrs. The expressions of CD11b and CD14 were (78.4±18.5)% and (18.7±2.9)% in control vs (83.3±14.2)% and (20.4±2.1)% in cells fractions treated by 30 μg Fe/ml PEI-Fe₄O₄-MNPs. Clony-forming rates of SHI-1 cells labeled by 0, 20 , 50 μg Fe/ml PEI-Fe₄O₄-MNPs were (25.20±7.22)%, (25.93±13.15)%, (23.37±9.33)%, respectively. Differentiation and colony-forming potentials of labeled cells were similar with control in the certain range of PEI-Fe₄O₄-MNPs concentration.

Conclusion: SHI-1 cells were efficiently labeled by PEI-Fe₄O₄-MNPs with well biocompatibilities in proper range of concentration, the latter could be coupled with magnetic resonance imaging (MRI) to track cells in vivo.

目的 初步探讨利用聚乙烯亚胺包被的四氧化三铁（PEI-Fe₄O₄）磁性纳米颗粒进行磁共振细胞影像技术跟踪细胞生物学行为的可行性。方法 以人急性单核细胞白血病细胞株SHI-1为对象,用透射电镜观察PEI-Fe₄O₄磁性纳米颗粒能否被细胞内吞;用电感耦合等离子发射光谱仪及普鲁士蓝染色观察影响细胞磁性纳米颗粒载量的因素;用CCK-8法检测PEI-Fe₄O₄对SHI-1细胞增殖的影响;用流式细胞术（FCM）检测PEI- Fe₄O₄磁性纳米颗粒对细胞分化的影响;以甲基纤维素半固体培养基培养法检测PEI-Fe₄O₄对细胞集落形成能力的影响。以未经PEI-Fe₄O₄磁性纳米颗粒处理的SHI-1细胞作为对照组。结果 PEI-Fe₄O₄磁性纳米颗粒能成功被SHI-1细胞内吞,细胞内磁性纳米颗粒载量与其起始浓度（以纳米颗粒中的铁离子浓度代表PEI-Fe₄O₄浓度,即μg Fe/ml）及与细胞共培养的时间呈正相关,随细胞分裂传代而减少。以5~100 μg Fe/ml PEI-Fe₄O₄处理细胞,60~100 μg Fe/ml各组对细胞增殖抑制率高于5~50 μg Fe/ml各组,差异具有统计学意义（P<0.05）;FCM检测对照组细胞CD11b和CD14分子的表达分别为（78.4±18.5）%和（18.7±2.9）%,佛波醇酯（TPA）处理后增至（92.1±6.5）%和（20.8±2.3）%,PEI-Fe₄O₄标记后的细胞CD11b和CD14分子的表达分别为（83.3±14.2）%和（20.4±2.1）%,TPA处理后增至（95.8±3.3）%、（21.0±6.9）%,实验组和对照组间在TPA处理前后的CD11b和CD14表达差异无统计学意义（P>0.05）;0、20、50 μg Fe/ml PEI-Fe₄O₄处理24 h后的SHI-1细胞集落形成率分别为（25.20±7.22）%、（25.93±13.15）%和（23.37±9.33）%,各组间差异无统计学意义（P>0.05）。结论 PEI-Fe₄O₄磁性纳米颗粒可高效率标记SHI-1细胞,在5~50 μg Fe/ml范围内,与SHI-1细胞具有很好的生物相容性,不影响SHI-1细胞的生物学行为,可作为磁共振细胞影像技术的标记物。

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