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Comment
. 2013 Oct;67(4):348-50.
doi: 10.1016/j.jinf.2013.05.002. Epub 2013 May 16.

Antibodies induced by receptor-binding domain in spike protein of SARS-CoV do not cross-neutralize the novel human coronavirus hCoV-EMC

Lanying Du, Cuiqing Ma, Shibo Jiang
Comment

Antibodies induced by receptor-binding domain in spike protein of SARS-CoV do not cross-neutralize the novel human coronavirus hCoV-EMC

Lanying Du et al. J Infect. 2013 Oct.
No abstract available

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Figures

Figure 1
Figure 1
Cross-reactivity and cross-neutralization activity of SARS-CoV S-RBD-specific antibodies against hCoV-EMC. (A) Reactivity of SARS-CoV S-RBD-specific mAbs with RBD and/or S1 protein of hCoV-EMC and SARS-CoV as detected by ELISA. Conf I–VI, Group A–E, and linear mAbs represent the mAbs targeting the conformational and linear epitopes in RBD of SARS-CoV S protein, respectively. HA-7 mAb specific to hemagglutinin (HA) of H5N1 influenza virus was used as the negative control. The data are presented as mean A450 ± standard deviation (SD) of duplicate wells. Neutralization of SARS-CoV S-RBD-specific mAbs (B) and SARS-CoV S-RBD protein-vaccinated mouse antisera (C) against hCoV-EMC and SARS-CoV infection by pseudovirus neutralization assay. The data are presented as mean percentages of neutralization ± SD of duplicate wells.
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