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. 2013:2013:273086.
doi: 10.1155/2013/273086. Epub 2013 Apr 18.

Biological Insights into Therapeutic Protein Modifications throughout Trafficking and Their Biopharmaceutical Applications

Xiaotian Zhong  1 , Jill F Wright
Affiliations

Biological Insights into Therapeutic Protein Modifications throughout Trafficking and Their Biopharmaceutical Applications

Xiaotian Zhong et al. Int J Cell Biol. 2013.

Abstract

Over the lifespan of therapeutic proteins, from the point of biosynthesis to the complete clearance from tested subjects, they undergo various biological modifications. Therapeutic influences and molecular mechanisms of these modifications have been well appreciated for some while remained less understood for many. This paper has classified these modifications into multiple categories, according to their processing locations and enzymatic involvement during the trafficking events. It also focuses on the underlying mechanisms and structural-functional relationship between modifications and therapeutic properties. In addition, recent advances in protein engineering, cell line engineering, and process engineering, by exploring these complex cellular processes, are discussed and summarized, for improving functional characteristics and attributes of protein-based biopharmaceutical products.

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Figures

Figure 1
Figure 1
Classification of therapeutic protein modifications along trafficking pathways.
See this image and copyright information in PMC

References

    1. Hammarsten O. Zur Frage, ob das Casein ein einheitlicher Stoff sei. Zeitschrift für Physiologische Chemie. 1883;7:227–273.
    1. Tagliabracci VS, Engel JL, Wen J, et al. Secreted kinase phosphorylates extracellular proteins that regulate biomineralization. Science. 2012;336:1150–1153. - PMC - PubMed
    1. Kornfeld S. Foreword from first edition. In: Varki A, Cummings RD, Esko JD, et al., editors. Essentials of Glycobiology. 2nd edition. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press; 2009. - PubMed
    1. Krishna RG, Wold F. Post-translational modification of proteins. Advances in Enzymology and Related Areas of Molecular Biology. 1993;67:265–298. - PubMed
    1. Seo J, Lee KJ. Post-translational modifications and their biological functions: proteomic analysis and systematic approaches. Journal of Biochemistry and Molecular Biology. 2004;37(1):35–44. - PubMed