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. 2013 Oct;32(10):1050-4.
doi: 10.1097/INF.0b013e31829bb0b9.

Genotypic diversity and mixed infection in newborn disease and hearing loss in congenital cytomegalovirus infection

Sunil K Pati  1 , Swetha Pinninti, Zdenek Novak, Nazma Chowdhury, Raj K Patro, Karen Fowler, Shannon Ross, Suresh Boppana; NIDCD CHIMES Study Investigators
Collaborators, Affiliations

Genotypic diversity and mixed infection in newborn disease and hearing loss in congenital cytomegalovirus infection

Sunil K Pati et al. Pediatr Infect Dis J. 2013 Oct.

Abstract

Background: Congenital cytomegalovirus (cCMV) is a common congenital infection and a leading nongenetic cause of sensorineural hearing loss (SNHL). CMV exhibits extensive genetic variability, and infection with multiple CMV strains (mixed infection) was shown to be common in congenital CMV. The role of mixed infections in disease and outcome remains to be defined.

Methods: Genotyping of envelope glycoproteins, UL55 (gB), UL73 (gN) and UL75 (gH), was performed on saliva specimens of 79 infants from the ongoing CMV and Hearing Multicenter Screening (CHIMES) Study and on blood and urine specimens of 52 infants who participated in natural history studies at the University of Alabama at Birmingham. Genotyping of UL144 and US28 was also performed in the CHIMES cohort. The association of individual genotypes and mixed infection with clinical findings at birth and SNHL was examined.

Results: Thirty-seven of 131 infants (28%) were symptomatic at birth and 26 (20%) had SNHL at birth. All known genotypes of UL55, UL75, UL73 and US28 were represented, and no particular genotype was associated with symptomatic infection or SNHL. UL144 subtype C was more common in symptomatic infants but not associated with SNHL. Mixed infection was observed in 59 infants (45%) and not associated with symptoms (P = 0.43) or SNHL at birth (P = 0.82). In the cohort of 52 infants with long-term hearing outcome, mixed infection at birth was not predictive of SNHL.

Conclusions: Mixed infection is common in infants with congenital CMV but is neither associated with symptomatic infection nor associated with SNHL.

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Conflict of interest statement

Conflict of interest: No authors have any conflict of interest to disclose.

Figures

Figure 1
Figure 1
The distribution of CMV UL55, UL73 and UL75 genotypes in saliva specimens from 131 infants with congenital CMV infection. All seven UL73 genotypes (gN-1, gN-2, gN-3a, gN-3b, gN-4a, gN-4b, gN-4c), all five UL55 genotypes (gB-1, gB-2, gB-3, gB-4, gB-5), and both UL75 genotypes (gH-1 and gH-2) were seen.
Figure 2
Figure 2
The distribution of CMV UL144 and US28 genotypes in saliva specimens from 79 CHIMES infants with congenital CMV infection. All five UL144 genotypes (A, B, C, AB and AC) all four known US28 genotypes (A, B, C, and D) and a new US28 type ‘E’ (in 2 infants) were seen.
See this image and copyright information in PMC

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