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. 2013 May;19(5):790-2.
doi: 10.3201/eid1905.121062.

Novel molecular type of Clostridium difficile in neonatal pigs, Western Australia

Michele M Squire  1 , Glen P Carter, Kate E Mackin, Anjana Chakravorty, Torbjörn Norén, Briony Elliott, Dena Lyras, Thomas V Riley
Affiliations

Novel molecular type of Clostridium difficile in neonatal pigs, Western Australia

Michele M Squire et al. Emerg Infect Dis. 2013 May.

Abstract

Clostridium difficile causes neonatal enteritis in piglets; strains of PCR ribotype 078 are most commonly identified. We investigated C. difficile prevalence in piglets in Australia and isolated a novel strain with a unique pathogenicity locus. In a mouse infection model, this strain produced more weight loss than did a ribotype 078 strain.

Keywords: Australia; C. difficile; Cdiff; Clostridium difficile; DNA; PCR; animals; bacteria; diarrhea; domestic; enteritis; piglets; pigs; prevalence; ribotyping; sequence analysis; veterinary; zoonoses.

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Figures

Figure 1
Figure 1
Structure of pathogenicity locus (PaLoc) and flanking regions in Clostridium difficile strains AI35 and VPI10463. Boxes indicate open reading frames; arrows indicate direction of transcription. Encoded genes are indicated above the arrows. Figure not drawn to scale.
Figure 2
Figure 2
A) Survival and B) percentage of weight lost in mice over 4 days after infection with Clostridium difficile. Male C57/B6 mice were infected with C. difficile spores for strains M7404 (triangles), JGS6133 (078) (diamonds), or A135 (circles); phosphate-buffered saline (squares) was used as control. Error bars in panel B indicate SEM.
See this image and copyright information in PMC

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