Identification by 16S rRNA gene sequencing of an Actinomyces hongkongensis isolate recovered from a patient with pelvic actinomycosis

Abstract

A case of Actinomyces hongkongensis pelvic actinomycosis in an adult woman is described. Conventional phenotypic tests failed to identify the Gram-positive bacillus isolated from a fluid aspirate of a pelvic abscess. The bacterium was identified by 16S rRNA gene sequencing and analysis using the SmartGene Integrated Database Network System software.

Fig 1

Axial computed tomography scan of the pelvis demonstrating a bilocular perivaginal abscess (arrows).

Fig 2

Neighbor-joining tree of 523 bp of the 16S rRNA gene sequences of the pelvic abscess isolate (GenBank accession no. KC962413) and Actinomyces reference strains. Branch support is recorded at the nodes as a percentage of 1,000 bootstrap iterations. GenBank accession numbers are shown in parentheses.