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Case Reports
. 2013 Aug;51(8):2721-3.
doi: 10.1128/JCM.00509-13. Epub 2013 May 22.

Identification by 16S rRNA gene sequencing of an Actinomyces hongkongensis isolate recovered from a patient with pelvic actinomycosis

A N Flynn  1 , C A Lyndon, D L Church
Affiliations
Case Reports

Identification by 16S rRNA gene sequencing of an Actinomyces hongkongensis isolate recovered from a patient with pelvic actinomycosis

A N Flynn et al. J Clin Microbiol. 2013 Aug.

Abstract

A case of Actinomyces hongkongensis pelvic actinomycosis in an adult woman is described. Conventional phenotypic tests failed to identify the Gram-positive bacillus isolated from a fluid aspirate of a pelvic abscess. The bacterium was identified by 16S rRNA gene sequencing and analysis using the SmartGene Integrated Database Network System software.

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Figures

Fig 1
Fig 1
Axial computed tomography scan of the pelvis demonstrating a bilocular perivaginal abscess (arrows).
Fig 2
Fig 2
Neighbor-joining tree of 523 bp of the 16S rRNA gene sequences of the pelvic abscess isolate (GenBank accession no. KC962413) and Actinomyces reference strains. Branch support is recorded at the nodes as a percentage of 1,000 bootstrap iterations. GenBank accession numbers are shown in parentheses.
See this image and copyright information in PMC

References

    1. Clinical and Laboratory Standards Institute 2008. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing. Approved standard MM18-A. CLSI, Wayne, PA
    1. Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24:1596–1599 - PubMed
    1. Woo PC, Lau SK, Teng JL, Tse H, Yuen KY. 2008. Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clin. Microbiol. Infect. 14:908–934 - PubMed
    1. Simmon KE, Croft AC, Petti CA. 2006. Application of SmartGene IDNS software to partial 16S rRNA gene sequences for a diverse group of bacteria in a clinical laboratory. J. Clin. Microbiol. 44:4400–4406 - PMC - PubMed
    1. Woo PC, Fung AM, Lau SK, Teng JL, Wong BH, Wong MK, Hon E, Tang GW, Yuen KY. 2003. Actinomyces hongkongensis sp. nov. a novel Actinomyces species isolated from a patient with pelvic actinomycosis. Syst. Appl. Microbiol. 26:518–522 - PubMed

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