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. 1990 Aug;10(8):4427-30.
doi: 10.1128/mcb.10.8.4427-4430.1990.

Hierarchic and cooperative binding of the rat liver nuclear protein C/EBP at the hepatitis B virus enhancer

R Dikstein  1 , O Faktor, Y Shaul
Affiliations

Hierarchic and cooperative binding of the rat liver nuclear protein C/EBP at the hepatitis B virus enhancer

R Dikstein et al. Mol Cell Biol. 1990 Aug.

Abstract

We used the enhancer-binding protein C/EBP as a model to study the nature and the complexity of interaction of an enhancer-binding protein with its target DNA. We found that bacterially expressed C/EBP binds the hepatitis B virus enhancer at multiple sites in a hierarchic and cooperative manner. At low concentrations, only the E element is occupied, but at higher concentrations, additional sites are filled including a site that binds EP, a crucial enhancer-activating protein. This pattern of C/EBP binding may explain the concentration-dependent effect of C/EBP on enhancer activity.

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