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. 2012 Sep;18(3):340-3.
doi: 10.4103/0971-6866.107989.

Analysis of loss of heterozygsity effect on thyroid tumor with oxyphilia cell locus in familial non medullary thyroid carcinoma in Iranian families

Hasti Atashi Shirazi  1 , Mehdi Hedayati, Maryam Sadat Daneshpour, Abdollah Shafiee, Fereidoun Azizi
Affiliations

Analysis of loss of heterozygsity effect on thyroid tumor with oxyphilia cell locus in familial non medullary thyroid carcinoma in Iranian families

Hasti Atashi Shirazi et al. Indian J Hum Genet. 2012 Sep.

Abstract

Material and methods: 22 nuclear families (78 persons including 12 patients) with papillary and follicular tumors were selected in a period of six months from Milad hospital. Five microsatellite markers (D19S413, D19S391, D19S916, D19S568, D19S865) on 19p13.2 were selected for genetic analysis. Genomic DNAs was extracted; PCR and polyacrylamide gel electrophoresis method were used for variation detection.

Results: The results show that 5.4% of the follicular carcinomas and 17.9% of the papillary carcinomas presented LOH at recognition sites. LOH of Papillary carcinoma detected about 13.9% and follicular carcinoma 7.2% in this study. The frequency of informative cases was not similar for each marker: D19S413 (41.1%)[1], D19S391 (12.5%), D19S916 (10.7%), D19S568 (1.8%) and D19S865 (3.6%). Loss of hetrozygosity in D19S413 predicts the relation between variation in this region and the disease.

Discussion: Our findings showed an average of 13.9% LOH in FNMTC cases. Among the five major microsatellites, D19S413 was the most informative for LOH analysis of FNMTC.

Keywords: Familial non medullary thyroid carcinoma; Iran; loss of heterozygosis; thyroid tumor with oxyphilia cell.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
LOH analysis of informative cases by five microsatellite markers in patients and their families and also papillary and follicular carcinoma in the study
Figure 2
Figure 2
Acrylamide gel, PCR result and detected LOH; a. chromosomes 19p13.2 sample 1: control, sample 2: patient, sample 3: her husband and samples 4, 5, and 6 are her children; b. chromosomes 19p13.2 sample 2: patient; 3: his wife, 1: control and 4, 5 and 6 are his children
Figure 3
Figure 3
Location of 5 markers on chromosome 19 with their Marshfield regions
Figure 4
Figure 4
Pedigree of family papillary and follicular, with haplotype analysis in the region of linkage to susceptibility to thyroid cancer and TCO, on chromosome 19. Circles represent females, and squares represent males; crossed symbols denote cases of thyroid cancer. Critical recombination in individuals II-2, II-3 and II-4 define a 1 - 1-0 - 1-1 haplotype that is co- inherited with the disease and that is not shared by the unaffected family members
See this image and copyright information in PMC

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