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. 2012 Jul;36(3):291-7.
doi: 10.5142/jgr.2012.36.3.291.

Enzymatic Transformation of Ginsenoside Rb1 by Lactobacillus pentosus Strain 6105 from Kimchi

Se-Hwa Kim  1 , Jin-Woo Min, Lin-Hu Quan, Sungyoung Lee, Dong-Uk Yang, Deok-Chun Yang
Affiliations

Enzymatic Transformation of Ginsenoside Rb1 by Lactobacillus pentosus Strain 6105 from Kimchi

Se-Hwa Kim et al. J Ginseng Res. 2012 Jul.

Abstract

Ginsenoside (ginseng saponin), the principal component of ginseng, is responsible for the pharmacological and biological activities of ginseng. We isolated lactic acid bacteria from Kimchi using esculin agar, to produce β-glucosidase. We focused on the bio-transformation of ginsenoside. Phylogenetic analysis was performed by comparing the 16S rRNA sequences. We identified the strain as Lactobacillus (strain 6105). In order to determine the optimal conditions for enzyme activity, the crude enzyme was incubated with 1 mM ginsenoside Rb1 to catalyse the reaction. A carbon substrate, such as cellobiose, lactose, and sucrose, resulted in the highest yields of β-glucosidase activity. Biotransformations of ginsenoside Rb1 were analyzed using TLC and HPLC. Our results confirmed that the microbial enzyme of strain 6105 significantly transformed ginsenoside as follows: Rb1→gypenoside XVII, Rd→F2 into compound K. Our results indicate that this is the best possible way to obtain specific ginsenosides using microbial enzymes from 6105 culture.

Keywords: Ginsenoside; Lactobacillus pentosus; Panax ginseng; Transformation.

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Figures

Fig. 1.
Fig. 1.. Phylogenetic tree based on 16S rRNA gene sequences showing the phylogenetic relationships between strain 6105 and related species.
Fig. 2.
Fig. 2.. Effect of temperature (A) and pH (B) on the transformation of ginsenoside Rb1 by crude enzyme strain 6105. CK, compound K; ST, standard.
Fig. 3.
Fig. 3.. HPLC assay of transformation of ginsenoside Rb1 to compound K by crude enzyme strain 6105.
Fig. 4.
Fig. 4.. Effects of carbon source supplementation on the transformation of ginsenoside Rb1 by crude enzyme of strain 6105. N, without elicitor; C, cellobiose; G, glucose; L, lactose; S, sucrose, H, hesperidin; T, soluble starch; St, standard; N-T, reaction with suspension culture; N’-T’, reaction with crude enzyme precipitated by acetone.
Fig. 5.
Fig. 5.. The conversion pathway of ginsenoside Rb1 using crude enzyme strain 6105.
See this image and copyright information in PMC

References

    1. Duo D, Jin L, Chen Y. Advances and prospects of the study on chemical constituents and pharmacological activities of Panax ginseng. J Shenyang Pharm Univ. 1999;16:151–156.
    1. Yuan CS, Wang CZ, Wicks SM, Qi LW. Chemical and Pharmacological Studies of Saponins with a Focus on American Ginseng. J Ginseng Res. 2010;34:160–167. doi: 10.5142/jgr.2010.34.3.160. - DOI - PMC - PubMed
    1. Park JH., Kim JM, Han SB, Kim NY, Surh YJ, Lee SK, Kim ND, Park MK. A new processed ginseng with fortified activity. In: Advances in Ginseng Research – Proceedings of the 7th International Symposium on Ginseng; 1998. Korean Society of Ginseng; Seoul: 1998. pp. 146–159.
    1. Kim MK, Lee JW, Lee KY, Yang DC. Microbial conversion of major ginsenoside Rb1 to pharmaceutically active minor ginsenoside Rd. J Microbiol. 2005;43:456–462. - PubMed
    1. Kwon SW, Han SB, Park IH, Kim JM, Park MK, Park JH. Liquid chromatographic determination of less polar ginsenosides in processed ginseng. J Chromatogr A. 2001;921:335–339. - PubMed

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