Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Aug;177(2):768-71.
doi: 10.1016/0042-6822(90)90546-4.

Map location of lactate dehydrogenase-elevating virus (LDV) capsid protein (Vp1) gene

E K Godeny  1 , D W Speicher, M A Brinton
Affiliations

Map location of lactate dehydrogenase-elevating virus (LDV) capsid protein (Vp1) gene

E K Godeny et al. Virology. 1990 Aug.

Abstract

Lactate dehydrogenase-elevating virus (LDV) is currently classified within the Togaviridae family. In an effort to obtain further information on the characteristics of this virus, we have begun to sequence the viral RNA genome and to map the virion structural protein genes. A sequence of 1064 nucleotides, which represents the 3' terminal end of the genome, was obtained from LDV cDNA clones. A 3' noncoding region of 80 nucleotides followed by two complete open reading frames (ORFs) were found within this sequence. The two ORFs were in different reading frames and overlapped each other by 11 nucleotides. One ORF encoded a protein of 170 amino acids and the other ORF, located adjacent to the 3' noncoding region of the viral genome, encoded a 114 amino acid protein. Thirty-three N-terminal residues were sequenced directly from purified LDV capsid protein, Vp1, and this amino acid sequence mapped to the ORF adjacent to the 3' noncoding region. The presence of overlapping ORFs and the 3' terminal map position of Vp1 indicate that LDV differs significantly from the prototype alpha togaviruses.

PubMed Disclaimer

References

    1. Westaway E.G., Brinton M.A., Gaidamovich S.Ya., Horzinek M.C., Igarashi A., Kaariainen L., Lvov D.K., Porterfield J.S., Russell P.K., Trent D.W. Togaviridae. Intervirol. 1985;24:125–139. - PubMed
    1. Westaway E.G., Brinton M.A., Gaidamovich S.Ya., Horzinek M.C., Igarashi A., Kääriäinen L., Lvov D.K., Porterfield J.S., Russell P.K., Trent D.W. Flaviviridae. Intervirol. 1985;24:183–192. - PubMed
    1. Collett M.S., Moennig V., Horzinek M.C. J. Gen. Virol. 1989;70:253–266. - PubMed
    1. Spaan W.J.M., den Boon J.A., Bredenbeek P.J., Chirnside E.D., Noten A.F.H., Snijder E.J., de Vries A.A.F., Horzinek M.C. In: New Aspects of Positive Strand RNA Viruses. Brinton M.A., Heinz F.X., editors. ASM Press; Washington, DC: 1990. pp. 12–19.
    1. Brinnton-Darnell M., Plagemann P.G.W. J. Virol. 1975;16:420–433. - PMC - PubMed

Publication types

Associated data

LinkOut - more resources