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. 2013 May 30:13:251.
doi: 10.1186/1471-2334-13-251.

Emergence of 16S rRNA methylase-producing Acinetobacter baumannii and Pseudomonas aeruginosa isolates in hospitals in Vietnam

Tatsuya Tada, Tohru Miyoshi-Akiyama, Yasuyuki Kato, Norio Ohmagari, Nozomi Takeshita, Nguyen Viet Hung, Doan Mai Phuong, Truong Anh Thu, Nguyen Gia Binh, Nguyen Quoc Anh, Tran Thi Thanh Nga, Pham Hong Truong, Phan Thi Xuan, Le Thi Anh Thu, Nguyen Truong Son, Teruo Kirikae

Emergence of 16S rRNA methylase-producing Acinetobacter baumannii and Pseudomonas aeruginosa isolates in hospitals in Vietnam

Tatsuya Tada et al. BMC Infect Dis. .

Abstract

Background: 16S rRNA methylase-producing Gram-negative bacteria are highly resistant to all clinically important aminoglycosides. We analyzed clinical strains of 16S rRNA methylase-producing Acinetobactor baumannii and Pseudomonas aeruginosa obtained from clinical isolates in medical settings in Vietnam.

Methods: From 2008 to 2011, 101 clinical strains of A. baumannii and 15 of P. aeruginosa were isolated from patients in intensive care units (ICUs) in two medical settings in Vietnam. Antimicrobial susceptibilities were determined using the microdilution method and epidemiological analysis was performed by pulsed-field gel electrophoresis and MLST. Genes encoding the 16S rRNA methylases, OXAs and CTX-Ms were analyzed by PCR and sequence analysis.

Results: 16S rRNA methylase-producing Gram-negative pathogens were detected in two hospitals in Vietnam. Of the 101 clinical isolates of A. baumannii and the 15 of P. aeruginosa isolated from two ICUs in these hospitals, 72 (71.3%) were highly resistant to amikacin, arbekacin and gentamicin, with MICs greater than 1,024 mg/L. The 16S rRNA methylases ArmA and RmtB were produced by 61 and 9 isolates of A. baumannii, respectively, and RmtB was produced by 2 isolates of P. aeruginosa. Moreover, 52 of the A. baumannii isolates producing 16S rRNA methylases harbored both blaOXA-23-like and blaOXA-51-like genes. Most A. baumannii isolates producing 16S rRNA methylase obtained in hospital A in Hanoi were ST91 and ST231, whereas most from hospital B in Ho Chi Minh City were ST136, ST195, and ST254.

Conclusions: Gram-negative bacteria producing the 16S rRNA methylases ArmA and RmtB are emerging in medical settings in Vietnam. A. baumannii isolates in northern and southern regions of Vietnam may be of different lineages.

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Figures

Figure 1
Figure 1
PFGE pattern and MLST analysis of 101 Acinetobacter baumannii isolates. Eight clusters (I-VIII) with more than 70% similarity were identified. Isolates harboring armA (●) and rmtB (○) are shown in the column on the right.
Figure 2
Figure 2
PFGE pattern and MLST analysis analysis of 15 Pseudomonas aeruginosa isolates. Eight clusters with more than 70% similarity were detected. Two clusters (I and II) with more than 70% similarity were identified. Isolates harboring rmtB (○) are shown in the column on the right.
See this image and copyright information in PMC

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