Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Jun;3(6):464-9.
doi: 10.1016/S2221-1691(13)60097-0.

In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves

Mohammad Sekendar Ali  1 , Mohammad Ruhul Amin, Chowdhury Mohammad Imtiaz Kamal, Mohammad Aslam Hossain
Affiliations

In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves

Mohammad Sekendar Ali et al. Asian Pac J Trop Biomed. 2013 Jun.

Abstract

Objective: To study the leaves of Adiantum philippense L. for their antioxidant, cytotoxicity and thrombolytic activities and to perform phytochemical evaluation.

Methods: In-vitro antioxidant activity of extract was studied using DPPH radical scavenging, reducing power, total phenol and total flavonoid content determination assays. The cytotoxic activity was determined using brine shrimp lethality bioassay, thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis.

Results: The antioxidant activity of the extracts was found promising. The reducing power of this crude extract increase with the increase of concentration; IC50 values of DPPH scavenging activity was (140.00±0.86) µg/mL as compared to ascorbic acid [IC50 (130.00±0.76) µg/mL]; Total phenol and total flavonoids content were (148.26±0.24) mg/mL and (163.06±0.56) mg/mL respectively. In cytotoxicity assay the LC50 values of the sample was (106.41±0.78) µg/mL where as for standard vincristin sulphate was (08.50±0.24) µg/mL as a positive control and the extract shows (12.86±1.02)% clot lytic whereas standard streptokinase shows (30.86±0.44% clot lytic activity in thrombolytic assay. The phytochemical evaluation indicates the presence of chemical constituents including carbohydrates, alkaloids, saponins, glycosides, flavonoids.

Conclusions: This study shows that the methanol extract of leaves of Adiantum philippense L. has bioactivity but further compound isolation is necessary to confirm the activities of individual compounds.

Keywords: Adiantum philippense L.; Antioxidant; Cytotoxicity; Phytochemical evaluation.

PubMed Disclaimer

Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. The free radical scavenging activity of A. philippense and ascorbic acid by DPPH.
Results are mean±SEM of three measurements.
Figure 2.
Figure 2.. Reducing capacity of A. philippense extract.
Values are mean±SEM of three experiments.
Figure 3.
Figure 3.. Toxicity assay of A. Philippense on brine shrimp.
The results are expressed as mean±SEM of three measurements.
Figure 4.
Figure 4.. The clot lysis activity of A. Philippense extract and streptokinase.
All results are mean±SEM of three consecutive experiments.
See this image and copyright information in PMC

References

    1. Harman D. Origin and evolution of the free radical theory of aging: a brief personal history 1954-2009. Biogerontol. 2009;10(6):773–781. - PubMed
    1. Clancy D, Birdsall J. Flies, worms and the free radical theory of ageing. Ageing Res Rev. 2013;12(1):404–412. - PubMed
    1. Karthikeyan R, Manivasagam T, Anantharaman P, Balasubramanian T, Somasundaram ST. Chemopreventive effect of Padina boergesenii extracts on ferric nitrilotriacetate (Fe-NTA)-induced oxidative damage in Wistar rats. J Appl Phycol. 2011;23(2):257–263.
    1. Chatterjee S, Lardinois O, Bhattacharjee S, Tucker J, Corbett J, Deterding L, et al. et al. Oxidative stress induces protein and DNA radical formation in follicular dendritic cells of the germinal center and modulates its cell death patterns in late sepsis. Free Radic Biol Med. 2011;50(8):988–999. - PMC - PubMed
    1. Bob Shepard. Birmingham, Alabama: UAB News; 2011. Antioxidant may prevent alcohol-induced liver disease. [Online] Available from: http://www.uab.edu/news/latest/item/1181-antioxidant-may-prevent-alcohol....

Publication types

LinkOut - more resources