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. 2013 May 30;8(5):e64607.
doi: 10.1371/journal.pone.0064607. Print 2013.

Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature

Hongmei Sun  1 , Guanhua Xue, Chao Yan, Shaoli Li, Ling Cao, Yi Yuan, Hanqing Zhao, Yanling Feng, Liqiong Wang, Zhaoyang Fan
Affiliations

Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature

Hongmei Sun et al. PLoS One. .

Abstract

Mycoplasma pneumoniae is one of the major respiratory bacterial pathogens that cause pneumonia in humans. Multiple-locus variable-number tandem-repeat analysis (MLVA) is currently the most discriminative method for typing M. pneumoniae strains. To better understand the epidemic of M. pneumoniae-related pneumonia in pediatric patients in Beijing, China, we performed MLVA analysis on 118 specimens collected during an epidemic from 2010-2012. Eleven distinct MLVA types were identified, including four novel types. There was no obvious association of macrolide resistance with any of the genotypes. Considering the instability of VNTR locus Mpn1, we propose an amended MLVA nomenclature system based on the remaining four VNTR loci.

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Conflict of interest statement

Competing Interests: The authors have declared that no competing interests exist.

Figures

Figure 1
Figure 1. Positive rate of M. pneumoniae detection from January 2010–December 2012.
Figure 2
Figure 2. Minimum spanning trees for Mycoplasma pneumoniae detected in Beijing, 2010 (n = 20, A), 2011 (n = 21, B), and 2012 (n = 75, C).
Each circle represents a unique genotype. The size of each circle illustrates the proportion of specimens with that genotype. The smallest circle in each tree represents one specimen. Two adjacent circle connected by a line had a single locus variant.
See this image and copyright information in PMC

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