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Review
. 2013 May 30;7(5):e2161.
doi: 10.1371/journal.pntd.0002161. Print 2013.

Potential non-invasive urine-based antigen (protein) detection assay to diagnose active visceral leishmaniasis

Claudia Abeijon  1 , Antonio Campos-Neto
Affiliations
Review

Potential non-invasive urine-based antigen (protein) detection assay to diagnose active visceral leishmaniasis

Claudia Abeijon et al. PLoS Negl Trop Dis. .
No abstract available

PubMed Disclaimer

Conflict of interest statement

I have read the journal's policy and have the following conflicts: CA is an employee of DetectoGen Inc. and has no ownership or ownership option in the company. ACN is a consultant for DetectoGen Inc.

Figures

Figure 1
Figure 1. Antigen detection capture ELISA for the identification of the proteins Li-isd1, Li-txn1, and Li-ntf2 in urine of VL patients and controls.
Urine specimens were from VL patients and normal, healthy control subjects. The following predetermined capture antibodies concentrations were used to coat the ELISA plates: antigen affinity-purified IgY anti-Li-isd1, 100 ng/well; antigen affinity-purified rabbit anti-Li-txn1 antibodies, 875 ng/well; and purified rabbit IgG anti-Li-ntf2 antibody (2,000 ng/well), or the combination of the three antibodies/well. Samples from VL patients (n = 20) were from Teresina, PI, Brazil. Control samples were from healthy individuals from countries where VL is endemic who were living in the United States. Dashed lines represent the cutoff values calculated as described in the text. These are representative results of at least three experiments performed at different times with the same urine samples and same capture ELISA.
Figure 2
Figure 2. Specificity of the capture ELISA assembled with a combination of antibodies specific to Li-isd1, Li-txn1, and Li-ntf2 antigens.
Urine specimens were from patients with cutaneous leishmaniasis (CL) (n = 10), Chagas disease (CD) (n = 8), schistosomiasis (SC) (n = 14), or tuberculosis (TB) (n = 10). Assay performed using capture ELISAs assembled all three antibodies in a single well as described in the legend for Figure 1. Dashed line represents the cutoff values, which was calculated using the average of the ODs obtained from the urine specimens from 20 normal, healthy control subjects plus 3 SDs.
See this image and copyright information in PMC

References

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