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. 1990 Jul 11;18(13):3983-6.
doi: 10.1093/nar/18.13.3983.

Constructing DNA by polymerase recombination

A A Yolov  1 , Z A Shabarova
Affiliations
Free PMC article

Constructing DNA by polymerase recombination

A A Yolov et al. Nucleic Acids Res. .
Free PMC article

Abstract

Polymerase-mediated recombination based on DNA polymerase chain reactions (PCRs) has been used to carry out directed joining at a present point of two DNA fragments initially contained in a plasmid and a single-stranded synthetic DNA. The process includes copying of these fragments by PCR with generation of an overlapping homologous region. Such overlap of 12 base pairs in length was found to be sufficient to provide further DNA joining also by use of PCR.

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