Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Jun;5(3):271-81.
doi: 10.2217/epi.13.24.

Association between birth weight and DNA methylation of IGF2, glucocorticoid receptor and repetitive elements LINE-1 and Alu

Affiliations

Association between birth weight and DNA methylation of IGF2, glucocorticoid receptor and repetitive elements LINE-1 and Alu

Heather H Burris et al. Epigenomics. 2013 Jun.

Abstract

Aim: We examined the association between birth weight and methylation in the imprinted IGF/H19 loci, the nonimprinted gene NR3C1 and repetitive element DNA (LINE-1 and Alu).

Materials & methods: We collected umbilical cord venous blood from 219 infants born in Mexico City (Mexico) as part of a prospective birth cohort study and analyzed DNA methylation using pyrosequencing.

Results: Birth weight was not associated with DNA methylation of the regions studied. One of the CpG dinucleotides in the IGF2 imprinting control region (ICR)1 includes a potential C-T SNP. Among individuals with an absence of methylation at this site, probably due to a paternally inherited T allele, birth weight was associated with mean methylation status of both IGF2 ICR1 and ICR2. However, this association would not have survived adjustment for multiple testing.

Conclusion: While we did not detect an association between DNA methylation and birth weight, our study suggests a potential gene-epigene interaction between a T allele in the IGF2 ICR1 and methylation of ICRs of IGF2, and fetal growth.

PubMed Disclaimer

Figures

Figure 1
Figure 1. The IGF2 cluster and parent-of-origin imprinting patterns
DMR: Differential methylation region; ICR: Imprinting control region.
Figure 2
Figure 2. DNA methylation of the IGF2 imprinting control region 1 (H19 differential methylation region), CpG site 2
DMR: Differential methylation region; ICR: Imprinting control region.
Figure 3
Figure 3
Location of the rs10732516 SNP within the imprinting control region 1 assay of the IGF2 gene.

References

    1. Brodsky D, Christou H. Current concepts in intrauterine growth restriction. J Intensive Care Med. 2004;19(6):307–319. - PubMed
    1. Ross MG, Beall MH. Adult sequelae of intrauterine growth restriction. Semin Perinatol. 2008;32(3):213–218. - PMC - PubMed
    1. Gluckman PD, Hanson MA, Cooper C, Thornburg KL. Effect of in utero and early-life conditions on adult health and disease. N Engl J Med. 2008;359(1):61–73. - PMC - PubMed
    1. Gillman MW. Developmental origins of health and disease. N Engl J Med. 2005;353(17):1848–1850. - PMC - PubMed
    1. Horike S, Ferreira JC, Meguro-Horike M, et al. Screening of DNA methylation at the H19 promoter or the distal region of its ICR1 ensures efficient detection of chromosome 11p15 epimutations in Russell–Silver syndrome. Am J Med Genet A. 2009;149A(11):2415–2423. - PubMed

Website

    1. Mayo Clinic. [Accessed 1 February 2012];Beckwith–Wiedemann syndrome (BWS)/Russell–Silver syndrome (RSS) molecular analysis. www.mayomedicallaboratories.com/test-catalog/Specimen/61010.

Publication types

Substances