Spiroplasma as a model insect endosymbiont

Abstract

Members of the genus Spiroplasma are actively motile and helical bacteria of the class Mollicutes, which are associated with a variety of arthropods and plants. Some spiroplasmas cause female-biased sex ratios of their host insects as a result of selective death of the male offspring during embryogenesis. Several strains of male-killing spiroplasmas have been successfully transfected into Drosophila melanogaster by haemolymph injection and maintained in laboratory fly stocks. Spiroplasma-Drosophila endosymbiosis represents an ideal model system for analysing the molecular mechanisms underlying host-symbiont interactions. The infection dynamics exhibited by the symbiont within the host, the effects of external and environmental factors on the symbiotic association and symbiont interactions with the host's immune system have been investigated using this system. Comparisons between a male-killing Spiroplasma strain and its non-male-killing variant revealed that, in addition to different male-killing abilities, they also differed in infection dynamics and resistance to host innate immunity. It is currently unclear whether these different phenotypes are interconnected to each other. However, if so, such pleiotropy could facilitate our understanding of the genetic and molecular mechanisms of the endosymbiotic system.