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. 2013 May 20:2013:593029.
doi: 10.1155/2013/593029. Print 2013.

Isolation and Characterization of Mycoplasma mycoides Subspecies capri from Milk of Natural Goat Mastitis Cases

Vijay Kumar  1 , Rajneesh Rana, Somya Mehra, Pramod Kumar Rout
Affiliations

Isolation and Characterization of Mycoplasma mycoides Subspecies capri from Milk of Natural Goat Mastitis Cases

Vijay Kumar et al. ISRN Vet Sci. .

Abstract

Association of Mycoplasma mycoides subspecies capri (Mmc) with natural goat mastitis has been studied earlier largely by detecting the Mmc DNA using molecular methods. However, report on detection of cultivable Mmc isolates from natural goat-mastitis milk is still very rare. In this study, Mmc was isolated from milk samples (n = 171) of goats with or without clinical signs of mastitis. Mmc isolates were further characterized by biochemical and species-specific PCR methods. Intra species strain variation was also studied by 16S amplified rDNA restriction analysis (16S ARDRA). The study recovered a total of 6 Mmc isolates (3.5%). Three types of intraspecies variants among the recovered Mmc isolates were found by 16S ARDRA. The study concluded that Mmc may be an etiological agent of mycoplasmal mastitis in Indian goat herds.

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Figures

Figure 1
Figure 1
M. mycoides subsp. Capri-specific PCR exhibiting 195 bp amplicon. M: 100 bp DNA ladder; PC: positive control (M. mycoides subsp. capri PG-3); NC: negative control; Lanes 1–6: respective isolates.
Figure 2
Figure 2
Intra specific strain variation in 16S ARDRA profile among Mmc isolates. M: 100 bp DNA ladder; PG-3: M. mycoides subsp. capri PG-3; Lanes 1–6: respective isolates. The band, that is, 236 bp, 186 bp, 147 bp, and 105 bp are visible very faintly in lane number 6.
See this image and copyright information in PMC

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