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. 2013 Dec;158(12):2577-81.
doi: 10.1007/s00705-013-1754-z. Epub 2013 Jun 15.

Protection of Chinese painted quails (Coturnix chinensis) against a highly pathogenic H5N1 avian influenza virus strain after vaccination

Julia Sarkadi  1 , Mate Jankovics, Zoltan Kis, Jozsef Skare, Kinga Fodor, Eva Gonczol, Ildiko Visontai, Zoltan Vajo, Istvan Jankovics
Affiliations

Protection of Chinese painted quails (Coturnix chinensis) against a highly pathogenic H5N1 avian influenza virus strain after vaccination

Julia Sarkadi et al. Arch Virol. 2013 Dec.

Abstract

Chinese painted quails immunized with a single dose (6 μg HA) of inactivated H5N1 (clade 1) influenza vaccine NIBRG-14 and challenged with 100 LD50 of the heterologous A/Swan/Nagybaracska/01/06(H5N1) (clade 2.2) strain were protected, whereas unvaccinated quails died after challenge. No viral antigens or RNA were detected in cloacal swabs from immunized animals. Sera obtained post-immunization gave low titres in serological assays against the vaccine and the challenge viruses. Our results demonstrate the protective efficacy of the NIBRG-14 strain against the challenge virus and the usefulness of these small birds in protection studies of influenza vaccines.

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Fig. 1
Fig. 1
Protection of Chinese painted quails immunized with the NIBRG-14 (clade 1) vaccine against the highly pathogenic heterologous A/Swan/Nagybaracska/01/06(H5N1) (clade 2.2) strain. Sixteen birds vaccinated s.c. with a single dose of NIBRG-14 vaccine (6 HA/dose) were challenged in two groups of eight birds with 10 or 100 times the LD50 of the A/Swan/Nagybaracska/01/06(H5N1) strain at 3 weeks post-immunization. Sixteen unvaccinated birds were similarly challenged. Mortality was monitored on a daily basis for 14 days
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