Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Dec;158(12):2577-81.
doi: 10.1007/s00705-013-1754-z. Epub 2013 Jun 15.

Protection of Chinese painted quails (Coturnix chinensis) against a highly pathogenic H5N1 avian influenza virus strain after vaccination

Julia Sarkadi  1 , Mate Jankovics, Zoltan Kis, Jozsef Skare, Kinga Fodor, Eva Gonczol, Ildiko Visontai, Zoltan Vajo, Istvan Jankovics
Affiliations

Protection of Chinese painted quails (Coturnix chinensis) against a highly pathogenic H5N1 avian influenza virus strain after vaccination

Julia Sarkadi et al. Arch Virol. 2013 Dec.

Abstract

Chinese painted quails immunized with a single dose (6 μg HA) of inactivated H5N1 (clade 1) influenza vaccine NIBRG-14 and challenged with 100 LD50 of the heterologous A/Swan/Nagybaracska/01/06(H5N1) (clade 2.2) strain were protected, whereas unvaccinated quails died after challenge. No viral antigens or RNA were detected in cloacal swabs from immunized animals. Sera obtained post-immunization gave low titres in serological assays against the vaccine and the challenge viruses. Our results demonstrate the protective efficacy of the NIBRG-14 strain against the challenge virus and the usefulness of these small birds in protection studies of influenza vaccines.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Protection of Chinese painted quails immunized with the NIBRG-14 (clade 1) vaccine against the highly pathogenic heterologous A/Swan/Nagybaracska/01/06(H5N1) (clade 2.2) strain. Sixteen birds vaccinated s.c. with a single dose of NIBRG-14 vaccine (6 HA/dose) were challenged in two groups of eight birds with 10 or 100 times the LD50 of the A/Swan/Nagybaracska/01/06(H5N1) strain at 3 weeks post-immunization. Sixteen unvaccinated birds were similarly challenged. Mortality was monitored on a daily basis for 14 days
See this image and copyright information in PMC

References

    1. Boon ACM, Finkelstein D, Zheng M, Liao G, Allard J, Klumpp K, Webster R, Peltz G, Webby RJ (2011) H5N1 influenza virus pathogenesis in genetically diverse mice is mediated at the level of viral load. MBio 2(5): e00171–11. doi:10.1128/mBio/mBio.00171-11 - PMC - PubMed
    1. Bright RA, Carter DM, Crevar CJ, Toapanta FR, Steckbeck FD, Cole KS, Kumar NM, Pushko P, Smith G, Tumpey TM, Ross TM. Cross-clade protective immune respomses to influenza viruses with H5N1 HA and NA elicited by an influenza virus-like particle. PLoS ONE. 2008;3(1):e1501. doi: 10.1371/journal.pone.0001501. - DOI - PMC - PubMed
    1. Committee for Proprietary Medicinal Products. 12 March 1997. Note for guidance on harmonisation of requirements for influenza vaccines. CPMP/BWP/214/96. European Agency for the Evaluation of Medicinal Products, London, UK. http://www.emea.europa.eu/pdfs/human/bwp/021496en.pdf
    1. Dybing JK, Schultz-Cherry S, Swayne DE, Suarez DL, Perdue ML. Distinct pathogenesis of Hong-Kong-origin H5N1 viruses in mice compared to that of other highly pathogenic H5 avian influenza viruses. J Virol. 2000;74:1443–1450. doi: 10.1128/JVI.74.3.1443-1450.2000. - DOI - PMC - PubMed
    1. European Committee for Proprietary Medicinal Products. Guideline on dossier structure and content for pandemic influenza vaccine marketing authorisation application (CPMP/VEG/4717/03). European Agency for the Evaluation of Medicinal Products, 2004, London, UK

Publication types

MeSH terms

LinkOut - more resources