doi: 10.1007/978-1-62703-493-7_9.
Human leukocyte antigen (HLA) typing by DNA sequencing
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- PMID: 23775737
- DOI: 10.1007/978-1-62703-493-7_9
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Human leukocyte antigen (HLA) typing by DNA sequencing
Methods Mol Biol.
2013.
Abstract
DNA sequencing is a powerful technique for identifying allelic variation within the human leukocyte antigen (HLA) genes. Sequencing is usually focused on the most polymorphic exons of the class I (HLA-A, -B, -C) and class II (HLA-DR, -DQ, and -DP) genes. These exons encode the antigen recognition site, the region of the HLA molecule that binds peptides and interacts with the T cell receptor for antigen and natural killer cell immunoglobulin-like receptors (KIR). Sanger sequencing of amplified DNA from each HLA gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a reference database.
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