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. 1990 Aug;87(15):5594-7.
doi: 10.1073/pnas.87.15.5594.

Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene

K Koike  1 , Y Urata, M Koike
Affiliations

Molecular cloning and characterization of human pyruvate dehydrogenase beta subunit gene

K Koike et al. Proc Natl Acad Sci U S A. 1990 Aug.

Abstract

A genomic clone encompassing the entire gene for the human pyruvate dehydrogenase beta subunit (PDH beta) has been isolated by screening a leukocyte genomic library with a nick-translated human foreskin fibroblast PDH beta cDNA probe. The 18-kilobase clone was characterized by restriction enzyme analysis, extensive DNA sequencing, and primer-extension analysis. The PDH beta structural gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8. The 5' flanking region of the PDH beta gene contains a "CAAT" consensus promoter sequence but no "TATA" sequence. Primer-extension analysis indicated that the PDH beta gene transcription start site is an adenine residue located 132 bases upstream from the initiation codon in exon 1.

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References

    1. J Mol Biol. 1975 Nov 5;98(3):503-17 - PubMed
    1. J Biochem. 1976 Jun;79(6):1273-85 - PubMed
    1. Adv Biophys. 1976;:187-227 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed

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