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. 2012 Jul;3(2):94-7.
doi: 10.4103/2229-4708.103883.

Validation of 1-methyl-2-phenylindole method for estimating lipid peroxidation in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg (9.)

Yasir Hasan Siddique  1 , Smita Jyoti, Falaq Naz, Mohammad Afzal
Affiliations

Validation of 1-methyl-2-phenylindole method for estimating lipid peroxidation in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg (9.)

Yasir Hasan Siddique et al. Pharm Methods. 2012 Jul.

Abstract

Background: A method using 1-methyl-2-phenylindole was developed for the estimation of lipid peroxidation in third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg (9). The method is specific for the estimation of malonaldehyde.

Materials and methods: The larvae were exposed to 0.0025, 0.025, 0.050, and 0.100 μl/ml of cyclophosphamide for 24 and 48 h. The homogenate was prepared of the larvae tissue explant and the absorbance was noted at 586 nm.

Results: A significant dose-dependent increase in the mean absorbance values was observed for both 24 and 48 h of exposure as compared to the untreated group.

Conclusions: On the basis of results obtained, it is suggested that the present method is more precise, accurate, and robust for the estimation of lipid peroxidation in the third instar larvae of transgenic D. melanogaster (hsp70-lacZ)Bg (9).

Keywords: 1-methyl-2-phenylindole; Drosophila melanogaster (hsp70-lacZ) Bg9; lipid peroxidation.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
Standard graph for the estimation of lipid peroxidation
Figure 2
Figure 2
Regression analysis for the standard graph
Figure 3
Figure 3
Lipid peroxidation in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ) Bg9 after the exposure of various doses of cyclophosphamide for 24 and 48 h of exposure
Figure 4
Figure 4
Regression analysis for the dose effect of cyclophosphamide on lipid peroxidation for the Drosophila melanogaster (hsp70-lacZ) Bg9 third instar larvae exposed for 24 h
Figure 5
Figure 5
Regression analysis for the dose effect of cyclophosphamide on lipid peroxidation for the Drosophila melanogaster (hsp70-lacZ) Bg9 third instar larvae exposed for 48 h
See this image and copyright information in PMC

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