Effects of naked gold nanoparticles on proinflammatory cytokines mRNA expression in rat liver and kidney

Abstract

The data on the biocompatibility of naked gold nanoparticles (GNPs) are scarce, and their interpretation is controversial. We studied the acute (1 day) and subchronic (5 days) effects of GNPs (10 and 50 nm diameter) on expression of interleukin-1 beta (IL-1 β ), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF- α ) in the liver and kidneys of rats. In the liver, the GNPs of both sizes (10 and 50 nm) significantly increased the cytokines gene expression on day 1 which was subsided on day 5; the GNPs of 50 nm size produced more severe inflammatory response as compared to smaller sized GNPs. In the kidney, the GNPs did not produce any significant change in the expression of IL-1 β . Although the gene expression of IL-6 and TNF- α was not affected by GNPs of 10 nm size, 50 nm GNPs significantly increased the expression of IL-6 and TNF- α in the kidneys of rats on day 1 after treatment which returned to normalcy on day 5. These findings indicate the possible immunocompatibility of medium sized GNPs as they caused only a transient acute phase increase in proinflammatory cytokines expression followed by their normalcy during the repeated exposure.

Figure 1

Time-course effects of gold nanoparticles (GNPs) of 10 and 50 nm diameter on proinflammatory cytokines gene expression in rat liver. *P < 0.05 and **P < 0.01 versus control group using Dunnett's test.

Figure 2

Time-course effects of gold nanoparticles (GNPs) of 10 and 50 nm diameter on proinflammatory cytokines gene expression in rat kidney. *P < 0.01 versus control group using Dunnett's test.