Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990;59(1-2):253-60.

Isolation and characterization of form specific DNA sequences of O. volvulus

K D Erttmann  1 , S E Meredith, B M Greene, T R Unnasch
Affiliations
  • PMID: 2378208

Isolation and characterization of form specific DNA sequences of O. volvulus

K D Erttmann et al. Acta Leiden. 1990.

Abstract

Maintenance of the control of O. volvulus in Africa would be assisted by the development of assays to identify the origin of outbreaks of transmission within the controlled regions. The isolation of pFS-1, a DNA sequence specific for forest form O. volvulus, has suggested that it might be possible to develop such an assay based on form specific DNA probes. The isolation and characterization of pSS-1, a clone which hybridized preferentially to savannah form O. volvulus is described here. DNA sequence analysis demonstrated that pSS-1 was a representative of a highly repeated sequence family of O. volvulus. Comparison of the sequence of pSS-1 to other known examples of this family has identified a region of pSS-1, designated pSS-1BT, which appeared to be specific for savannah form O. volvulus. Tests of isolates from different areas demonstrated that the specificity of pFS-1 and pSS-BT were not confined to a small geographic region. However, the close relationship of pFS-1 and pSS-BT to less specific members of the repeat family means that their specificity and sensitivity are affected by small changes in the conditions under which they are used.

PubMed Disclaimer

Publication types