Localization of DNA in Coxiella burnetii by post-embedding immunoelectron microscopy

Abstract

The deoxyribonucleic acid of Coxiella burnetii was detected with monoclonal antibodies against single-stranded and double-stranded DNA by post-embedding immunoelectron microscopy. The antibody labeled the nucleoid of the spore, the small cell variant (SCV), and the large cell variant (LCV). The DNA was segregated completely in both the spore and the mother cell. At the terminal stage of development of the spore, the loss of nuclear morphology in the mother cell was related to the reduction in the labeling of the nucleoid. The mother cell was therefore killed for the development and release of the spore.