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. 2013 Dec;158(12):2487-94.
doi: 10.1007/s00705-013-1767-7.

Genetic properties of endemic Chinese porcine epidemic diarrhea virus strains isolated since 2010

Xiao-meng Wang, Bei-bei Niu, He Yan, Dong-sheng Gao, Xia Yang, Lu Chen, Hong-tao Chang, Jun Zhao, Chuan-qing Wang

Genetic properties of endemic Chinese porcine epidemic diarrhea virus strains isolated since 2010

Xiao-meng Wang et al. Arch Virol. 2013 Dec.

Abstract

Acute diarrhea outbreaks caused by porcine epidemic diarrhea virus (PEDV) have been observed in various pig-breeding provinces of China since December 2010. Endemic strains of PEDV were isolated from different areas, and the complete genome sequences of 10 isolates were determined. Our objective in this study was to genetically characterize current Chinese field isolates of PEDV to better understand their epidemiology and genetic diversity. Sequence analysis showed that 10 post-2010 isolates shared high homology with each other and were always clustered together with the virulent DR13 strains (South Korea) and/or one earlier Chinese strain, CH-S, in phylogenetic analysis. All post-2010 isolates possessed common sequence changes in each gene. Our results suggest that current Chinese PEDV isolates originated from either South Korean and/or Chinese ancestors that underwent some genetic variation, thereby forming a new PEDV genotype in China.

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Figures

Fig. 1
Fig. 1
Amplification of PEDV-specific fragments from CH/ZMDZY/11-infected Vero cells. S, spike gene (4161 bp); ORF3, open reading frame 3 (675 bp); E, envelope gene (231 bp); M, membrane gene (681 bp); N, nucleoprotein gene (1326 bp)
Fig. 2
Fig. 2
Partial peptide sequence alignment of the S genes of the 10 current Chinese PEDV strains (AJ1102, BJ-2011-1, CH-FJND-3-2011, CH GD-01, CH-ZMDZY-11, GD-1, GD-A, GD-B, LC, ZJCZ-4) and selected reference PEDV strains (European strain CV777, Chinese isolates CH-S and LZC, and Korean isolates SM98, virulent DR13, and attenuated DR13)
Fig. 3
Fig. 3
Phylogenetic analysis of the 10 current Chinese PEDV strains and reference isolates based on the nucleotide sequences of ORF1a/b (a), S (b), ORF3 (c), E (d), M (e), and N (f). Phylogenetic trees were constructed using MEGA 5.02 software. ●, current Chinese strains; ○, earlier Chinese isolates; △, reference strains. GenBank accession numbers of all strains examined in this study are listed in Table 1
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